Evaluation of the Antimicrobial Activity of Strombosia grandifolia Hook.f. ex Benth Extract Hand Sanitizer Formulation
DOI:
https://doi.org/10.35516/jjps.v16i1.1063Keywords:
Strombosia grandifolia, antimicrobial activity, phytochemical screening, hand sanitizerAbstract
The hand is an easy agent for the spread and transmission of pathogens and community-acquired infections. This study aims at evaluating the antimicrobial activity of Strombosia grandifolia hand sanitizer compared with a commercially available alcohol-based hand sanitizer. Water, methanol, and ethyl acetate extract from the leaf of Strombosia grandifolia was used to formulate hand sanitizer. The antimicrobial activity of the hand sanitizers was carried out against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Candida albicans and analyzed with one-way ANOVA. The sanitizers were smooth with a chilly feel on the skin. Aqueous and methanol extract sanitizer had a significantly better antimicrobial activity when compared with ethyl acetate extract with the rank order of susceptibility of the microorganisms as Staphylococcus aureus > Bacillus subtilis > Pseudomonas aeruginosa > Escherichia coli > Candida albicans. The incorporation of the extract synergized the antimicrobial activity of alcohol-based hand sanitizers.
References
(1) Wani S.N., Bhalerao K.P., Ranaware V.P. et al.Formulation and evaluation of herbal sanitizer. Int. J. Pharm. Tech. Res. 2013; 5:40-43.
(2) Grace F.X., Diarsika K.V., Jorky A. et al.Polyherbal hand sanitizer formulation and evaluation. Indian J. Pharm. Pharmacol. 2015; 2:143-144.
(3) Pickering A.J., Davis J. and Alexandria B.B. Efficacy of alcohol based hand sanitizer on hands soiled with dirt and cooking oil. J. Water Health. 2011; 9:429- 433.
(4) Stanley C.N., Alobari V.B. and Ezealisiji K.M. Formulation and evaluation of the effectiveness of a novel hand sanitizer using Pleurotus ostreatus oyster mushroom extract. Int. J. Pharm. Res. Rev. 2017; 6:7-15.
(5) Pickering A.J., Alexandria B., Boehm A.B. et al. Efficacy of waterless hand hygiene compared with handwashing with soap: A field study in Dar es Salaam, Tanzania. Am. J. Trop. Med. Hyg. 2010; 82:270-278.
(6) Thombare A.M., Babaso V.U., Tushar P.H.et al. Formulation and evaluation of a novel herbal hand sanitizer. Indo. Am. J. Pharm. Res. 2015; 5:483-488.
(7) Nwabueze S.A., Amah C.C., Azuike E.C.et al. Ebola viral disease prevention: Perception of secondary school students in two districts in Anambra State, Nigeria. Issues Sci. Res. 2016; 1:1-9.
(8) WHO. Guidelines on hand Hygiene in health care: First global patient safety challenge clean care is safer care. Geneva, 2009.
(9) Ekalu A., Ayo R.G., Habila J.D.et al. In vitro antimicrobial activity of lignan from the stem bark of Strombosia grandifolia Hook.f. ex Benth. Bull. Natl. Res. Cent. 2019; 43:1-7.
(10) Trease A., Evans W.C. Pharmacognosy London: Balliene Tindiall,1989; 13th edition.
(11) Adeleye O.A., Babalola C.O., Femi-Oyewo M.N.et al. Antimicrobial activity and stability of Andrographis paniculata cream containing shea butter. Nig. J. Pharm. Res. 2019; 15:9-18.
(12) WHO. Guide to local production: WHO- recommended hand rub formulations. Geneva, 2010.
(13) Bhide M.M. and Nitave S.A. Formulation and evaluation of polyherbal cosmetic cream. World J. Pharm. Pharm. Sci. 2016; 5:1527-1536.
(14) Sekar M. and Halim F.H. Formulation and evaluation of natural anti-acne cream containing Syzygium samarangense fruits extract. Annual Res. Rev. Bio. 2017; 17:1-7.
(15) Shashidi G.H., Aghighi S. and Karimi N.A. Antibacterial and antifungal survey in plants used in indigenous herbal-medicine of south east regions of Iran. J. Biol. Sci. 2004; 4:405–12.
(16) Cowan M.M. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 1999;12:64-82.
(17) Zablotowicz R.M., Hoagland R.E. and Wagner S.C. Effect of saponins on the growth and activity of rhizosphere bacteria. Springer, Boston, MA, 1996.
(18) Shimada T.J. Salivary proteins as a defense against dietary tannins. Chem. Ecol. 2006; 32:1149-63.
(19) Murugan Y., Wins J.A. and Murugan M. Antimicrobial and phytochemical evaluation of Cassia auriculata. Indian J. Pharm. Sci. 2013; 75:122–125.
(20) Alayo A.M., Femi-Oyewo M.N., Bakre L.G.,et al. Antimicrobial studies of the leaf extract of Argemone mexicana and its ointment formulation. West Afr. J. Pharm. 2015; 26:33-40.
(21) Braun-Falco O. and Korting H.C. Normal pH value of human skin. Hautarzt 1986; 37:126-129.
(22) Zlotogorski A. Distribution of skin surface pH on the forehead and cheek of adults. Arch. Dermatol. Res. 1987; 279:398–401.
(23) Ali H., Alkowni R., Jaradat N.et al.. Evaluation of phytochemical and pharmacological activities of Taraxacum syriacum and Alchemilla arvensis. Jordan j. pharm. sci, 2021;14:457-472
(24) Allam H., Bennaceur M., Ksouri R., et al.Identification of phenolic compounds and assessment of the antioxidant and antibacterial properties of Thymelaea microphylla Coss. et Dur. from Western Algerian Sahara (Ain-Sefra Province) . Jordan j. pharm. sci. 202; 13:363- 376
(25) Gonelimali F.D., Lin J., Miao W., et al.Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Front. Microbiol. 2018; 9:1639.
(26) Golin A.P., Choi D. and Ghahary A. Hand sanitizers: A review of ingredients, mechanisms of action, modes of delivery, and efficacy against coronaviruses. Am. J. Infect. Control. 2020; 48:1062-1067.
