Comparison between the Efficacy of Nigella Sativa Aqueous Extract and Its Oil on Methimazole-induced Hypothyroidism in Albino Mice
DOI:
https://doi.org/10.35516/jjps.v16i1.1072Keywords:
Aqueous extract, Nigella sativa, FT4, FT3, TSH, HypothyroidismAbstract
Objective: This study was conducted to evaluate the impact of both aqueous extract of Nigella sativa and its oil in treating the hypothyroid induced by methimazole in female of white mice in order to determine which one of them is more effective in treating of hypothyroidism, aiming to use it as a natural treatment instead of the chemical treatments which have dangerous side effects.
Materials and Methods: The study included 40 female white mice dived into 4 groups 10 mice each one. Group 1 (control): orally administered and treated with distilled water. Group 2 (HYP): orally treated with methimazole at a dose 0.05-mg/kg/ body weight/day for 3 weeks. Group 3 (NSE): the hypothyroidism was induced as in group 2, then treated orally with aqueous Nigella sativa extract at a dose of 400mg/kg/body weight/ day for 4 weeks. Group 4 (NSO): the hypothyroidism was induced as group 2, then treated orally with Nigella sativa oil at a dose of 1 ml/kg/body weight/ day for 4 weeks.
Results: This study showed that both NSE and NSO caused a significant decreasing p<0.05 of TSH level and a significant increase of FT3 level. Also, NSO caused increasing of FT4 level, while the increase of FT4 was un-significant under NSE treatment.
Conclusions: The efficacy of NSO is higher than the NSE in treating hypothyroidism.
References
Mashayekh-sardoo H., Rezaee R. and Karimi Gh. Nigella sativa (black seeds) safety: an overview. Asian Biomed. 2020; 14(4):127-137.
Tekeli, A. Nutritional value of black cumin (Nigella sativa) meals as an Alternative protein source in poultry. J.anim.sci.adv. 2014;(4):797-806.
Islam M., Guha B., Hosen S., et al. Nigellalogy: Areview on Nigella sativa. MoJ Bioequtiv. 2017; 3(6):00056
Nickavar B., Mojab F., Javidnia K. et al. Chemical composition of the fixed and volatile oils of nigella sativa l. from Iran, Zeitschrift Fur Naturforschung C. 2003; 58(9-10):629-631.
B of essential oil from nigella sativa L. seeds cultivated in Syria. International journal of chemical research.2015; 8(10): 76-82
Nergiz C. and Otles S. Chemical composition of Nigella sativa L. seeds.Food chemistry. 1993;48: 259-261
Salem M. Immunomodulatory and therapeutic properties of the Nigella sativa L. seeds. International Immunopharmacology .2005;5: 1749–1770.
Al-Ghamdi M. The anti-inflammatory, analgesic and antipyretic activity of nigella sativa. Journal of Ethnopharmacology. 2001; 76: 45-48 .
.Fadel H. Effects of extracts of some medical plants in physiological parameters in animals. Tishreen university journal For Research and Scientific Studies-Biological Sciences Series .2011;33(4): 67-80
.Bamosa A., Kataabi H., Lebda F., Efect of Nigella sativa seeds on Glycemic control of patints with type 2 Diabits Mellitus. Indian journal physiol pharmacol. 2010; 54(4):344-354.
Juma F. and Abd-Alrhman H. the effects of Nigella sativa oil administration on some physiological values of reproductive aspect of rats. Iraqi.J.vet.Med.2011;35(2): 52-60.
Barakat E., ELWakeel L. and HAGAG R.Effects of Nigella sativa on outcome of hepoststis c in Egypt. World journal of Gastrointrology. 2013;19(16): 25-29
KHonche A., Husine H., Ghalomeani M. et al. Standardized Nigella sativa seed oil ameliorates hepatic steatosis,aminotransferase and lipid levels in non-alcoholic fatty liver disease: Arandomized, double-blind and placebo-controlled trail. Journal of Ethnopharmacology. 2019;234:106-111.
.Cholmanezahed Z., Shakeri Z., Saadat S. et al. clinical and experimental effects of nigella sativa and its constituents on respiratory and allergic disords. Avicenna journal of phyto med. 2019;9(3):195-212.
Mader, S.S. Under Standing Human Anatomy & physiology. Fifth Edition, The Mc Graw-Hill companies,2004, p:191.
Espostio T., Lucariello A. and Tammaro P. the effects of curcuma and its adjuvant on TPC1 thyroid cell line. Chimico-Biological intermactions.2019; 2335:112-118
Gayton, Arthur; Hull, John. Reference in Physiology of Physician Gaiton Hall, nine edition - translation Sadiq, al-Hilali. Academia International, Beirut, 1997, P 1295.
Sharif S., Elmahdi B., Mohammed A. and Mohammed A.H. The effects of nigella sativa ethanolic extract on thyroid function in normal and alloxan–induced diabetic rats. Thyroid Research and practice. 2012; 9(2): 48-52.
Shariatifar A., Riazi M. and Jahormy H. Effects of nigella sativa extract o fatigue, blood biochemical parameters and thyroid function in male mice. Chines Midicine.2014;5:16-21
Farhangi M., Dehghan P., Tagmiri S.et al. The effect of Nigella sativa on thyroid function,serum vascular endothelial growth factor(VEGF),Nesfate-1 and a thropometrec features in patients with Hashimoto's thyroiditis. BMC complementary and alternative medicine. 2016;16:471
Herndez M., Lopez R., Abanas R. et al. Antimicrobial activity of visnea mocanera leaf extracts. J. Ethnopharmacology. 1994; 41:115-119.
Abd Elazeem A., Mohammed M. and Hassan E. Effect of Experimentally induced hypothyroidism on the parotid gland of adult male albino rate and possible of role of thyroid hormone supplementation. British Journal of Science. 2016;14(1):20-36.
Hayat N., Tahhir M., Munnir B. et al. Effect of methimazole induced hypothyroidism on histological Characteristics of parotid gland of albino rate. Journal of Ayub Medical College Abbottabad. 2010; 22(3):22-27.
Caki M., Korac A. and Davidovic V. Methimazole induced hypothyroidism: Effect on body weight and histological characteristics of thyroid gland . J ugslov Med Biohem .2004; 23(2):143-147
Ebrhim I. The effect of nigella sativa extracts in the activity off thyroid gland in male rats. AL-Kufa J.for biology.2015;7(1): 2311.
Abou-Zeina H., Nasr S. and Abedel-Azem. Effect of different dietary supplementation with antioxidants on Gene expression and blood Antioxidant markers as well as thyroid hormones statusin Gota Kids .Middle-East J.Sc.Res.2015;23(23):993-1004.
Khalawi A., Robai A., Khoja M. et al. Can Nigella sativa oil (NSO) Revres hypothyroid Status induced by PTU in rat ?. Biochemical and Histological. Studies life science Journal. 2013; 10(2):802-811.
Al Jehani E., Alsaggf S., Ramadan W. et al. Neuroprotective effects of thymoquinon against cerebellar histopathological in propylthiouracil induced hypothyroidism in adult rats.Tropical Journal of pharmaceutical Resarche. 2017; 16(5): 1029-1037.
Abd-El hack M., Al aghawane M., Farag M., et al. Nutritional Healthical and therapeutic Efficacy of black cumin (Nigella sativa ) in animal. poultry and Human.int.j.phamacology .2016; 12:232-248.
Tembhurni S., Feroze S., More B. et al. Areview on therapeutic potential of Nigella sativa (kalonji)seeds. J. Med. Plant. Res.2014;8(4):167-177.
Perveen R. therapeutic effects of black cumin (Nigella sativa).A systematic review. Progress in Nutrition.2019; 21: 40-49.
Deshpande U., Joseph l., Patwardhan U., et al. Effect of antioxidants (vitamins C, E ad turmeric extract) on methimazole induced hypothyroidism in rats. Indian Journal of Experimental Biology. 2002;40: 735-738.
Abdallah E., Goma A. and Sayed M. The effect of omega-3 on cognition in hypothyroid in adult male rats. Acta physiologica . 2014; 101(3): 326
Burman, S. and Chandra, G. A study on antibacterial efficacy of different extracts of Artocarpus Chama fruits and identification of bioactive compounds in most potent extract. Jordan j. pharm. sci. 2022; 15(1): 70-80.
Al Turfan A., Zinging E., Dariyerai N., et al. Cumustats M. Investigation of zinc and copper in methimazole induced hypothyroidism. folia Biological (praha). 2007;53: 183-188.
Glatter E., Eyble E., Kotyazova D. et al. Blood serum level of TSH and thyroid hormones and thyroid tissue content of iodine in rats under restricted selenium and iodine supply. Norsk Epidemologi .2014;11(2) 201-204.
Hamouda A., Sameeh M. and Shriurou R. Effect avocado (presea Amiricana), Cabbage, and Ginger (Zingiber officinall) on Rate liver thyroid injuries induced by CCL4. Journal of pharma and pharmacology. 2016;4: 108-118.
Latif A., Shoker R., Hragia B. Evaloution of effects levothroxin, and phenolic extracts of convolvulus arvensis on thyroid hormonal disorders induced in male mice by thiourea, Journal of College of Education. 2017;1(26): 522-530
Aboul-Fotoh G., Abou-elnour R. and Farag E. Histological Study on possible protective effects of curcumin on potassium dicrumate induced hypothyroidism in adult male albino rates. Egyptian journal histology. 2018;41(2): 220-235
Osman H., El-Mahdey, A., El-Sherbiny, E. Role of thyme extract against some biochemical Alterations induced by propylthiourascil in male rats. J. of food nutrition.2019; 7(11):794-800 .
Jemal, K., Sandeep, B.V., Polas, S., Phytochemical screening and in Vitro antioxidant activity analysis of allophylus serratus (ROXB) KuRZ. Jordan j. pharm. sci. 2022; 15(1).
