Phytochemical screening and in vitro antioxidant activity analysis of leaf and callus extracts of Allophylus serratus (ROXB) KURZ.
DOI:
https://doi.org/10.35516/jjps.v15i1.291Keywords:
Allophylus serratus, antioxidant activity, phytochemical screening, callus extractAbstract
Allophylus serratus is an important medicinal plant whose leaves, fruits, roots and stem have been in use in traditional medicines. The present study is aimed to carry out preliminary phytochemical analysis and to determine antioxidant activity of leaf and callus water, methanol, ethyl acetate, chloroform and petroleum ether extracts of A. serratus. The extracts were subject to various qualitative and quantitative tests for phytochemical analysis. An in vitro antioxidant activity of the extracts was investigated by DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS++,(2,2-azinobis(3-ethyl-benzothiozoline)-6-sulfonic acid) and FRAPS(Ferric reducing/ antioxidant power) assays. The qualitative phytochemical analysis confirmed the presence of alkaloids, phenols, flavonoids, glycocides, saponins, tannins, and steroids in both leaf and callus extracts. The quantitative analysis result showed higher yields of phenolics (104.73±4.3 mg) followed by flavonoids (69.63±6.35 mg) and soluble tannins (69.59±1.27mg) equivalent gallic acid/g of methanol, ethyl acetate and methanol, respectively. In case of callus extracts, the highest yield is phenols (54.42±6.59gm) followed by flavonoids (39.17±6.35 gm) equivalent gallic acid/g of methanol extracts. The antioxidant activity assays of both leaf and callus extracts demonstrated strong antioxidant activity which could be due to high content of phenolics and flavenoids. The methanol extract showed more antioxidant potential than the other extracts of both leaf and callus but leaf extracts show significant antioxidant activity than the callus extracts. Therefore, both leaf and callus extracts of Allophylus serratus contain phytochemicals that are potential source for antioxidant which suggested that the plant could serve as a source of useful drugs.
References
Akrout A, El-Jani H, Amouri S., Neffati M.. Screening of antiradical and antibacterial activities of essential oils of Artemisia campestris L., Artemisia herba-alba Asso. And Thymuscapitatus Hoff et Link. Growing wild in the southern of Tunisia. Recent Res Sc & Technol 2010; 2: 29-39
Bao J, Cay Y, Sun M, Warg G and Corke H Anthocyanins, flavonol and free radical scavenging activity of Chinese bayberry (Myricarubra) extracts and their color properties and stability. J Agric Food Chem. 2005; 53: 2327-2332.
Benzie IF, Strain JJ.. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem. 1996; 239:70–6.
Brand-williams W, Cuvelier ME and Berset C. Use of free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft and Technologie 1995; 28(1): 25-30.
Cespedes C.L, El-Hafidi M, Pavon N, Alarcon J. Antioxidant and cardioprotectiveactivities of phenolic extracts from fruits of Chilean blackberry Aristoteliachilensis (Elaeocarpaceae), Maqui. Food Chemistry 2008; 107: 820-829.
Chavan R. B. and Gaikwad D. K.. Antibacterial Activity of Medicinally Important Two Species of Allophylus- Allophylus cobbe (L.) Raeusch. and Allophylus serratus (Roxb.) Kurz. Journal of Pharmacognosy and Phytochemistry. 2013; 2,(1):1-7.
Devi, A. G., Shyma, T. B. and Raghavendra, M. P Plants used by the tribes for the treatment of digestive system disorders in Wayanad district, Kerala. Journal of Applied Pharmaceutical Science, 2013; 3 (08): 171-175
Duh, P. D.. Antioxidant activity of burdock (Arctiumlappalinne): its scavenging effect on free-radical and active oxygen. Journal of the American Oil Chemists’ Society, 1998; 75(4): 455–461.
Edeoga HO, Okwu DE, Mbaebie BO Phytochemical constituents of some Nigerian medicinal plants, Afr. J. Biotechnol. 2005; 4(7): 685-68.
Emad A. Ewais, Desouky S. A and Ezzat H. Eshazly Studies on callus induction, phytochemical constituents and antimicrobial activity of Solanumnigrum L. (Solanaceae). Nature and Science ; 2015;13(6).
Gawri S, and Upadhyay A,. A comparative study on the antimicrobial activity and the presence of phytochemicals in the petioles and callus of J. Curcus. J Phytology; 2012;4(3):18-2
Gordon M.C, David J.N.. Natural product drug discovery in the next millennium. Journal of Pharmaceutical Biology 2001; 39: 8-17.
Gupta A. K, Tandon N,. Reviews on Indian Medicinal Plants. Indian Council of Medical Research, New Delhi. 2004;2:90-92
Gyamfi MA, Yonamine M, Aniya Y. Free radical scavenging action of medicinal herbs from Ghana Thonningia sanguine on experimentally induced liver injuries. Gen. Pharmacol. 2002; 32 : 661-667.
Hagerman A.E, Riedl K.M, Jones G.A, Sovik K.N, Ritchard N.T, Hartzfeldt P.W, Riechel T.L.. High molecular weight plant polyphenolics (tannins) as biological antioxidants. Journal of Agricultural and Food Chemistry 1998 ;46: 1887- 1892.
Harborne JB. A Guide to modern techniques of plant Analysis. 1998; USA: Kluwer Academic Publisher.
Harborne, J.B., Phytochemical methods. 1973; London. Chapman and Hall, Ltd., 49-188.
Hazar Ali, Raed Alkowni, Nidal Jaradat and Motasem Masri. Evaluation of phytochemical and pharmacological activities of Taraxacum syriacum and Alchemilla arvensis. Jordan Journal of Pharmaceutical Sciences, 2021 ;14(4)
Kanungo S, Nahak G, Sahoo SL, Sahu RK.. Antioxidant Activity and phytochemical evaluation of Plumbagozeylanica Linn. in vivo and in vitro, Int J Pharm Pharm Sci., 2020; 4 (4) :522-526.
Karthikeyan A, Shanthi V, Nagasathaya A.. Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatodavasica L, International Journal of Green Pharmaceae, 2009; 3, 78-80.
Kubmarawa D, Ajoku G.A. Enworem N.M. and Okorie D.A,. Preliminary phytochemical and antimicrobial screening of 50 medicinal plants from Nigeria. African Journal of Biotechnology 2007; 6: 1690-1696.
Kumar M., Rawat P., Dixit P., Mishra D., Gautam A., Pandey R., Singh D., Chattopadhyay N., Maurya R.,. Anti-osteoporotic constituents from Indian medicinal plants. Phytomedicine 2010;17, 993–999.
Kumaravel R.S ,Maleeka Begumb S.F , Parvathib H. and SenthilKumarc M.,. Phytochemical screening and in vitro antioxidant activity of ethyl acetate leaf extracts of Pterocarpusmarsupium Roxb (Fabaceae). INT J CURR SCI, 2013; 9: E 46-55
Lister, E., & Wilson, P.. Measurement of total phenolics and ABTS assay for antioxidant activity (personal communication). 2001; Lincoln, New Zealand: Crop Research Institute.
Liu C.Z, Saxena P.K.. Saussurea medusa cell suspension cultures for flavonoid production. Methods Mol Biol; 2009;547: 53-59.
Mary Stephanie S. Carranza, Glenn G. Oyong , Virgilio C. Linis, Michael Dominic M. Ajero, Maria Carmen S. Tan. The Antioxidant and Antiproliferative Agents from the bark of Philippine Alstonia scholaris (L.) R. Br.(Apocynaceae). Jordan Journal of Pharmaceutical Sciences, 2020; 13(2).
Meziti A, Meziti H, Boudiaf K, Mustapha B, Bouriche H. Polyphenolic Profile and Antioxidant Activities of Nigella sativa Seed Extracts in vitro and in vivo. World Acad Sci Engn Technol 2012;(6): 4-24.
Mohsen, M.S. and Ammar, S.M.A,. Total phenolic contents and antioxidant activity of corn tassel extracts. Food Chem. 2008 ; 112: 595-598. 24.
Murashige T, Skoog F. A revised medium for rapid growth and bioassay with Tobacco tissue cultures. Physiologia Plantarum. 1962;15:460-497
Naczk M. and Shahidi F. Phenolics in cereals, fruits and vegetables: occurrence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis, 2006; 41(5), 1523– 1542.
Nahapetian A, Bassiri A. Changes in concentration and interrelationship of phytate, P, mg, Cu, Zn, in wheat during maturation. Journal of Agricultural and Food Chemistry.1974; 32:1179-1182
Namde H, and Wani M. Callus Induction Studies and Active Components and Antioxidant Activity Investigation from Leaves and Callus of Tecomastans L. Juss. Ex Kunth. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2014; 5(2) p. 604-610.
Oktay M, Culcin I, Kufrevioglu O.I, Determination of in vitro antioxidant activity of fennel (Foenniculumvulgare) seed extracts. Lebensm. Wiss. Technol. 2003; 36: 263-271.
Omoruyi, B.E., G. Bradley and A.J. Afolayan, Antioxidant and phytochemical properties of Carpobrotusedulis (L.) bolus leaf used for the management of common infections in HIV/AIDS patients in Eastern Cape Province. BMC Comple. Alt. Med., 2012; 12: 215. DOI: 10.1186/1472-6882-12-21.
Omoruyi, B.E., G. Bradley and A.J. Afolayan, Antioxidant and phytochemical properties of Carpobrotusedulis (L.) bolus leaf used for the management of common infections in HIV/ AIDS patients in Eastern Cape Province. BMC Comple. Alt. Med. 2012; 12: 215. DOI: 10.1186/1472-6882-12-215
Pallab K, Tapan B, Tapas P, Ramen K. Estimation of total flavonoids content (TPC) and antioxidant activities of methanolic whole plant extract of Biophytumsensitivum Linn. Journal of Drug Delivery and Therapeutics, 2013; 3(4): 33-37.
Patridge E, Gareiss P, Kinch M.S, Hoyer D. An analysis of FDA approved drugs: natural products and their derivatives. Drug Discov Today. 2016; 21:204–207.
Reddy B.S, Reddy B.P, Raghavulu S.V, Ramakrishna S, Venkateswarlu Y, Diwan P.V Evaluation of antioxidant and antimicrobial properties of Soymidafebrifuga leaf extracts. Phytotherapy Research 2008; 22: 943-947.
Sahai S., Commercialisation of indigenous knowledge and benefit sharing, Paper presented to UNCTAD Expert Meeting on Systems and National Experiences for Protecting Traditional Knowledge,., Innovations and Practices, 2000;Geneva, 30 October – 1 November.
Sandipta Ghosh , Tribeni Chatterjee , Anirban Sardar, Ishita Chatterjee, Arup Bose , Angana Dasgupta , Akash Malitha , Krishnendu Acharya. Antioxidant Properties and Phytochemical Screening of Infusion and Decoction Obtained from Three Cultivated Pleurotus Species: A Comparative Study. Jordan Journal of Pharmaceutical Sciences, 2020; 13(2).
Sanmuga Priya E., Senthamil Selvan P. and Tamilselvan R., Phytochemical Investigation of Allophylus serratus Kurz Leaves by UV and GC-MS Analysis. Research Journal of Phytochemistry, 2012; 6: 17-24
Singh R, Verma P.K, Singh G. Total phenolic, flavonoids and tannin contents in different extracts of Artemisia absinthium. J Intercult Ethnopharmacol. (2012); 1 (2): 101-104.
Singh R, Singh S, Kumar S, Arora S. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food and Chemical Toxicology 2007;45:1216-1223
Sofowora A. Medicinal Plants and Traditional Medicinal in Africa. 2nd Ed. Sunshine House, Ibadan, Nigeria: Spectrum Books Ltd; 1993. Screening Plants for Bioactive Agents; 1993; pp. 134–156
Supe U., Dhote, F. and Roymon, M. G.. A review on micro propagation of Withaniasomnifera – A medicinal plant. J. Agricultural Technology. 2011; 7(6): 1475-1483.
Tamuly C, Hazarika M, Bora J, Bordoloi M, Boruah M.P and Gajurel PR In vitro study on antioxidant activity and phenolic content of three Piper species from North East India. Journal of Food Science and Technology, 2013; DOI 10.1007/s 13197-013-1021-z.
Trease GE, Evans WC. A textbook of Pharma cognosy. 14th ed. London: Bailliere Tindall Ltd. 1996.
Trease, G.E and Evans, W.C Trease and Evans pharmacognosy 14th edition w.b Ssuvnder company limited, London 1996; Pp191-293.
Umashanker M, Shruti S. Traditional Indian herbal medicine used as antipyretic, antiulcer, anti diabetic and anticancer: a review. International Journal of Research in Pharmacy and Chemistry, 2011. 1(4); 1152-1159.
Verma K.C., Sapra S., Bagga A. and Verma S. K., Comparison of Various Active Ingredients Between Ex-situ and In-vitro Grown Plants of Withaniasomnifera L. and Cichoriumintybus L., The Bioscan, 2012; 7(2) : 347-351.
Wakdikar, S. Global health care challenge: Indian experiences and new prescriptions. Electronic Journal of Biotechnology. 2004; 7(3): 217-223.
WHO, World Health Report: Reducing risks, Promoting Healthy Life. 2002. Geneva: World Health Organization
Yildirim A., Mavi A., Oktay M., Kara A. A., Algur O. F., and Bilaloglu V. Comparison of antioxidant and antimicrobial activities of Tilia (Tiliaargentea Desf ex DC), sage (Salvia triloba L.), and Black tea (Camellia sinensis) extracts,” Journal of Agricultural and Food Chemistry, 2000; 48 (10): 5030–5034.
Zakaria Z.A, Rofiee M.S, The L.K, Salleh M.Z, Sulaiman M. R, Somchit M.N, Bauhinia purpurea leaves extracts exhibited in vitro antiproliferative and antioxidant activities. African journal of Biotechnology; 2011; 10(1): 65-74.
