Extracellular Synthesis of Magnesium Oxide at Nano and Bulk Scale: Antifungal Effect Against Candida albicans, Aspergillus niger
Anti fungal effect of MgO nano and bulk particles
DOI:
https://doi.org/10.35516/jjps.v16i4.864Keywords:
MgO nanoparticles, Biosynthesis, ficus indica, Antifungal activity, Candida albicans, Aspergillus nigerAbstract
The antifungal activity of magnesium oxide nanoparticles (MgO-NPs) prepared by the cactus plant (Opuntia ficus indica) at the nano and macro scale was evaluated against important human pathogens: Candida albicans and Aspergillus niger. The nanoparticles were characterized using UV–Vis, FTIR, DLS, EDX, and FESEM. UV–Vis analysis revealed a peak at 300 nm, and FT-IR analysis showed that the biomolecules played an important role in ions reduction, leading to the growth of MgO-NPs. A peak close to 400 cm−1 was observed, indicating Mg-O-Mg bonding. EDX analysis confirmed the presence of MgO-NPs. MgO-NPs were identified as nanospheres with diameters between 15.5 and 78.01 nm (average 42.28 nm), while MgO-Bulk was identified as macrospheres with lengths between 105.2 and 1313.9 nm (average 356.09 nm) using FESEM. Z-average sizes by DLS analysis were 46.04 nm and 377 nm. In vitro antifungal assays were evaluated using two methods: well diffusion and the microdilution method, and MgO-NPs showed the highest effect in both. The Minimum Inhibitory Concentration (MIC) for MgO-NPs was equal to 1.5 mg/mL and 6.25 mg/mL for C. albicans and A. niger, respectively.
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