بروتوكول فعال لنجاح اكثار نبات ال-Cardiospermum halicacabum: نبات زينة ذو قوى طبية ينمو في البيئة الأردنية
DOI:
https://doi.org/10.35516/JJAS.3717الكلمات المفتاحية:
C. halicacabum، زراعة الأنسجة، التجذير، نبات طبي، الأردن، التكاثر داخل الأنابيبالملخص
halicacabum هو نبات زينة معروف بفوائده الطبية الهامة. يتم استخدامه في علاج العديد من الأمراض، بما في ذلك الروماتيزم والاضطرابات العصبية ولدغات الثعابين. لكن الرعي الجائر وضعف إنبات البذور تشكل العوائق الرئيسية لتكاثر C. halicacabum واستدامته. يمكن تطبيق زراعة الأنسجة كتقنية فعالة لإكثار هذا النبات والحفاظ عليه. في هذا البحث، تم دراسة تأثير العديد من منظمات النمو ومستويات الرقم الهيدروجيني على تكاثر C. halicacabum. بالنسبة لتكاثر السويقات، تم اختبار منظمات النمو (بنزيل أمينو بورين (BAP) والزياتين والكاينتين) بمستويات مختلفة (0.0 و0.4 و0.8 و1.2 و1.6 و2.0 مجم / لتر) لتعزيز انتاج السويقات. وقد تبين أن البنزيل أمينو بورين (BAP) هو الهرمون المثالي لتكاثر السويقات عند (0.8 مجم/لتر). وفي الوقت نفسه، اظهر الرقم الهيدروجيني 6.0 اثرا إيجابيا على نمو النبات داخل الأنابيب. بالنسبة للتجذير العرضي، تم اختبار حمض إندول الأسيتيك (IAA) وحمض النفثالين الأسيتيك (NAA) وحمض إندول-3-بيوتريك (IBA) عند (0.0، 0.4، 0.8، 1.6،1.2 ،2.0 مجم/لتر). حيث تبين أن حمض إندول الأسيتيك هو الأفضل لتجذير C. halicacabum. كما تأقلمت معظم الشتلات في ظروف البيت الزجاجي. وبناء على النتائج فقد تم تطوير بروتوكول ناجح لإكثار C. halicacabum داخل الأنابيب. وهذا من شأنه أن يشجع على المزيد من العمل البحثي على C. halicacabum مثل البحث في مكوناته الطبية الفعالة وقوته المضادة للميكروبات.
التنزيلات
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