TLC Densitometry of Isoquercitrin Content in Kenikir Leaves (Cosmos caudatus Kunth.) and Tyrosinase Inhibitory and Anti-Propionibacterium acnes Bioactivity Assays

Nurwahidatul Arifa; Salsa Bila; Relin Yesika; Friardi  Ismed

doi:10.35516/jjps.v18i4.3317

Authors

Nurwahidatul Arifa Universitas Baiturrahmah
Salsa Bila Department of Clinical Pharmacy , Faculty of Health Sciences, Universitas Baiturrahmah, 25586, Padang, Indonesia
Relin Yesika 1 Department of Clinical Pharmacy , Faculty of Health Sciences, Universitas Baiturrahmah, 25586, Padang, Indonesia. 2 Department of Clinical Pharmacy , Faculty of Mathematics and Natural Sciences, Bengkulu University, 38119, Bengkulu, Indonesia.
Friardi Ismed The Laboratory of Natural Resource of Sumatra (LBS) and Faculty of Pharmacy, Universitas Andalas, 26163 Padang, Indonesia

DOI:

https://doi.org/10.35516/jjps.v18i4.3317

Abstract

Isoquercitrin is a 3-O-glucoside of quercetin identified in the TLC profiles of kenikir (Cosmos caudatus Knuth.) leaves extract and fractions. Quantification of isoquercitrin was effected by TLC-densitometry analysis with ethyl acetate/water/formic acid (10:1:1) mobile phase, developing with citroborate reagent and detecting at wavelength 366 nm. The linearity equation, y = 23.404x + 402.16, with a correlation coefficient value of 0.9925, indicated isoquercitrin levels of 15.7 and 24.6 mg/g in the total extract and ethanol fraction, respectively. The minimum inhibition concentration (MIC) for Propionibacterium acnes was determined using microdilution with the addition of iodonitrotetrazolium as a chromogenic agent. The antibacterial activity of the ethanol fraction against P. acnes was twice that of chloramphenicol, with MIC 0.625 mg/ml. Tyrosinase inhibition was evaluated by IC₅₀ spectrophotometrically. The ethanol fraction was more active in inhibiting tyrosinase enzyme than kojic acid, with IC₅₀ 6.803 µg/ml. C. caudatus ethanol fraction containing the flavonoid isoquercitrin has good tyrosinase inhibitory and anti-Propionibacterium acnes activity.

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