Genetic Diversity among Single, Double, And Triple Cross Hybrids in Beit Alpha Cucumber (Cucumis sativus)

Adel M.  Altamari; Muhanad W.  Akash; Mahmoud A.  Kasrawi

doi:10.35516/jjas.v18i1.103

Authors

Adel M. Altamari The University of Jordan, Amman 11942, Jordan.
Muhanad W. Akash The University of Jordan, Amman 11942, Jordan.
Mahmoud A. Kasrawi The University of Jordan, Amman 11942, Jordan

DOI:

https://doi.org/10.35516/jjas.v18i1.103

Keywords:

cucumber, genetic variation, AFLP, SSR, cluster analysis

Abstract

Genetic variation was investigated in fifteen single, eighteen three-way, and nine double hybrid crosses and their parents along with nine elite commercial cucumber hybrids. Six lines (NS5, NS9, NS17, NS29, NS31, and NS33) were used to develop these cross hybrids. Based on AFLP and SSR analysis, the studied cucumber genotypes have a narrow genetic base. The results also showed genetic differentiation compared to commercial varieties. Out of 1352 bands produced from 12 AFLP primer pairs, only 47 were polymorphic. Similarly, out of 26 SSR primers, only one primer was polymorphic. The highest heterozygosity index (0.6044), polymorphic information content value (0.5392), and marker Index (0.6044) were observed with M-CTT_E-AAG AFLP primer combination, while the highest effective multiplex ratio (2.8246) and resolving power (4.8421) were observed with M-CTC_E-AGC AFLP primer combination. Genetic distance estimation revealed clear distinctiveness of the studied genotypes with the control varieties. The cluster analysis by UPGMA showed two main clusters covering 84% of the studied genotypes grouped. This research shows that double and three-way hybrid crosses can be used in cucumber hybrid breeding.

Downloads

Download data is not yet available.

Author Biographies

Adel M. Altamari, The University of Jordan, Amman 11942, Jordan.

Department of Horticulture and Crop Science, The University of Jordan, Amman 11942, Jordan.

Muhanad W. Akash, The University of Jordan, Amman 11942, Jordan.

Department of Horticulture and Crop Science, The University of Jordan, Amman 11942, Jordan.

Mahmoud A. Kasrawi, The University of Jordan, Amman 11942, Jordan

Department of Horticulture and Crop Science, The University of Jordan, Amman 11942, Jordan

References

Akash M. W., N. Awad and M. A. Kasrawi. (2020). Genetic diversity among snake melon landraces (Cucumis Melo Var. Flexuosus) using molecular descriptors. Plant Biosystems.154, 2 ,206-212.

Akash, M.W. and Kang, M.S. (2009), Molecular Clustering and Interrelationships among Agronomic Traits of Jordanian Barley Cultivars. Journal of Crop Improvement, 24(1), 28-40.

Altamari, Adel M. M. A. Kasrawi, M. W. Akash, and T. M. AlAntary (2019). Performance of single, triple, and double-cross hybrids in Beit Alpha cucumber (Cucumis sativus L.) Fresenius Environmental Bulletin, 28(12a):10068-10076.

Amiryousefi, A., J. Hyvönen and P. Poczai, (2018). iMEC: Online marker efficiency calculator. Applications in Plant, 6(6)

Balestre, M., J.C. Machado, J.L. Lima., J.C. Souza, and NóbregaFilho, L. (2008), Genetic distance estimates among single cross hybrids and correlation with specific combining ability and yield in corn double-cross hybrids. Genetics and Molecular Research, 7(1), pp.65-73.

Danesh, M., M. Lotfi, and Azizinia, S. (2015), Genetic diversity of Iranian melon cultigens revealed by AFLP markers. International Journal of Horticultural Science and Technology, 2(1), pp.43-53.

Dice, R.L. (1945), Measures of the amount of ecological association between species. Ecology, 26: 297-302.

Fan, Z., M. D. Robbins, and Staub, J. E. (2006), Population development by phenotypic selection with subsequent marker-assisted selection for line extraction in cucumber (Cucumis sativus L.). Theoretical and Applied Genetics, 112: 843-855.

Fazio, G., J.E. Staub, and Chung, S.M. (2002), Development and characterization of PCR markers in cucumber. Journal of the American Society for Horticultural Science, 127(4): 545-557.

Horejsi, T., and Staub, J. E. (1999), Genetic variation in cucumber (Cucumis sativus L.) as assessed by random amplified polymorphic DNA1. Genetic Resources and Crop Evolution, 46(4): 337-350.

Hu, J. B., J.W. Li, L. J. Wang, L. J. Liu, and Si, S. W. (2011), Utilization of a set of high-polymorphism DAMD markers for genetic analysis of a cucumber germplasm collection. Acta Physiologiae Plantarum, 33(1): 227-231.

Hwang, J., K.A.N.G. Jumsoon, S.O.N. Byeonggu, K.I.M. Kwanghwan, and Younghoon, P.A.R.K. (2011), Genetic diversity in watermelon cultivars and related species based on AFLPs and EST-SSRs. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(2), p.285.

Innark, P., Khanobdee, C., Samipak, S., and Jantasuriyarat, C. (2013), Evaluation of genetic diversity in cucumber (Cucumis sativus L.) germplasm using agro-economic traits and microsatellite markers. Scientia Horticulturae, 162: 278-284.

Karakurt Y., Güvercin D., Önder S., Işler Ö. (2020), Assessment of genetic diversity in cucumber (Cucumis sativus L.) genotypes using morphological characters and AFLP analysis. KSU Journal of Agriculture and Nature, 23(3): 577-585.

Mliki, A., Staub, J. E., Zhangyong, S., and Ghorbel, A. (2003), Genetic diversity in African cucumber (Cucumis sativus L.) provides the potential for germplasm enhancement. Genetic Resources and Crop Evolution, 50(5): 461-468.

Nam, Y.W., J.R. Lee, K.H. Song, M.K. Lee, M.D. Robbins, S.M. Chung, J.E. Staub, and Zhang, H.B. (2005), Construction of two BAC libraries from cucumber (Cucumis sativus L.) and identification of clones linked to yield component quantitative trait loci. Theoretical and Applied Genetics, 111(1), pp.150-161.

Olfati, J. A., H. Samizadeh, G. Peyvast, B. Rabiei, and Khodaparast, S. A. (2012), Relationships between Hybrid Performance and Genetic Distance Revealed by Morphological and AFLP Marker in Cucumber. Plant Breeding and Seed Science, 65(1): 87-97.

Rohlf, F. J. (1998), Numerical Taxonomy and Multivariate Analysis System (NTSYS-PC), Exeter Publ, Setauket, N.Y.

Staub, J.E., S.M. Chung, and Fazio, G. (2005), Conformity and genetic relatedness estimation in crop species having a narrow genetic base: the case of cucumber (Cucumis sativus L.). Plant Breeding, 124(1), pp.44-53.

Torres, A.M., N.F. Weeden, and A. Martin. (1993), Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theoretical and Applied Genetics, 85:937–945.

Vos, P. R. Hogers, M. Bleeker, M. Reijans, T. Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper, M. and Zabeau, M. (1995), AFLP: a new technique for DNA fingerprinting. Nucleic Acids Resources, 23: 4407-4414.

Wang, H.Z., S.J. Li, X. Sun, and Guan, W., (2008), Analysis on the Genetic Relationship of Four Types of Cucumber Germplasms by AFLP Markers [J]. Acta Agriculturae Boreali-Sinica, 4, p.022.

Yilmaz, N., Y. A. Kacar, N. Sari, O. Simsek, and Solmaz, I. (2012), Evaluation of genetic relationships on the single, triple, and double-cross of melon (Cucumis melo var. cantalupensis) hybrids by SSR markers. Cucurbitaceae 2012, Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, University of Cukurova, ZiraatFakultesi, Antalya, Turkey, 15-18 October 2012, 544-550.