Molecular Characterization of Some Syrian Pomegranate (Punica granatum L.) Genotypes
Keywords:
Pomegranate, Syrian genotypes, genetic diversity, ISSRAbstract
Considering the high level of morphological diversity in Syrian pomegranate (Punica granatum L.) genotypes, this study was conducted to evaluate the genetic variation among five pomegranate genotypes using twenty inter simple sequence repeat (ISSR) markers. Twelve ISSR primers were successfully used as fingerprinting tools and amplified 137 DNA fragments out of which 78 were polymorphic (56.93%). Primers (17, NLSSR3, 16) showed the highest polymorphism percentage (90.41, 80.00, 76.47%, respectively) with the highest number of unique bands (6, 6, 9 respectively). Genotype C amplified the highest number of DNA fragments (115) and unique bands (13). Polymorphism information content (PIC) ranged from 0.449 to 0.768, while genetic diversity (GD) ranged from 0.56 to 0.80. The genetic distance ranged from 0.26 to 0.37. Some genotypes showed wide divergence (C and A; C and D), while genotypes A and B were closely related. The unweighted pair‐grouped method with arithmetic average (UPGMA)dendrogram grouped the genotypes into two clusters. These results indicated that the ISSR technique was sufficiently informative and powerful to assess genetic variability in pomegranate.
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Awamleh, H., Hassawi, D., Migdadi, H., Bruke, M. (2009). Molecular characterization of pomegranate (Punica granatum L.) landraces grown in Jordan using amplified fragment length polymorphism markers. Biotechnology 8(3): 316-322
Bedaf, M.T., Bahar, M., Sharifnabi, B., Yanchi, A. (2011). Evaluation of genetic diversity among Iranian pomegranate (Punica granatun 1.) cultivars, using ISSR and RAPD markers. Taxonomy and Biosystematics, 3rd year 8: 35-44.
Ercisli, S., Agar, G., Orhan, E., Yildirim, A., Hizarci, Y. (2007). Interspecific variability of RAPD and fatty acid composition of some pomegranate cultivars (Punica granattim L.) growing in Southern Anatolia Region in Turkey. Biochem Syst Ecol., 35:764-769
Hasnaoui, N., Buonamici, A., Sebastiané, F., Mars, M., Zhang, D., Vendramin, G.G. (2012). Molecular genetic diversity of Punica granatum L. (pomegranate) as revealed by microsatellite DNA markers (SSR). Gene, 493: 105-112.
Ismail, O.M., Younis, RA., Ibrahim, A.M. (2014). Morphological and molecular evaluation of some Egyptian pomegranate cultivars. African Journal of Biotechnology, 13(2): 226-237.
Jbir, R., Zehidi, S., Hasnaoui, N., Ben Dhiaf, A., Marsand, M., Sahli, H.A. (2012). Microsatellite polymorphism in Tunisian pomegranate (Punica granatum L.) cultivar genotyping and identification. Biochem Syst Ecol., 44: 27-35.
Mars, M. (2000). Pomegranate plant material: Genetic resources and breeding a review. Options Mediterraneenes: Series A, Seminaries Mediterranees, 42: 55-62.
Melgarejo, P., Martinez, J.J., Hernandez, F., Martinez, R., Legua, P., Oncina, R., Martinez Murcia, A. (2009). Cultivar identification using 18S-28S rDNA intergenic spacer-RFLP in pomegranate (Punica granatum L.). Sci Hort., 120: 500-503.
Moslemi, M., Zahravi, M., Khaniki, G.B. (2010). Genetic diversity and population genetic structure of pomegranate (Punica granatum L.) in Iran using AFLP markers. Sci Hortic, 126:441 447.
Narzary, D., Mahar, K.S., Rana, T.S., Ranade, S.A. (2009). Analysis of genetic diversity among wild pomegranate in Western Himalayas using PCR methods. Sci Hortic., 121:237 242.
Sarkhosh, A., Zamani, Z., Fatahi, R., Ebadi, A. (2006). RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) landraces. Sci Hort., 111: 24-29.
Seeram, N.P., Henning, S.M., Zhang, Y., Suchard, M., Li, Z., Heber, D. (2006). Pomegranate juice ellagitannin metabolites present in human plasma and some persist in urine for up to 48 hours. J. Nutr., 136(10):2481-2485.
Shrikant, S.M. (2015). Molecular characterization of pomegranate cv. Bhagwa, and its closely related genotypes. Ph.D. Thesis, Department of horticulture, Postgraduate Institute. Maharashtra. India. 93p
Singh, S.K., Meghwal, P.R., Pathak, R., Gautam, R., Kumar, S. (2013). Genetic diversity in Punica granatum revealed by nuclear rRNA, internal transcribed spacer and RAPD polymorphism. National Academy Science Letters, 36(2): 115-124.
Stover, E., Mercure, E.W. (2007). The Pomegranate: A new look at the fruit of paradise. HortScience, 42: 1088-1092.
Syrian Statistic Group (2010).
http://moaar.gov.sy/main/archives/920.
Wang, Z., Weber, J.L., Zhong, G., Tanksley, S.D. (1994). Survey of plant short tandem DNA repeats. Theoretical and Applied Genetics 88: 1-6.
Zahra, N., Ali, F, Saeed, H.R., Masoud, S., Somayeh, G.B., Ali, M., Seyed, Z.A. (2012). Genetic variation among lranian pomegranates (Punica granatum L using RAPD, ISSR, and SSR markers. Aust J Crop Sci., 6(2): 268-275.
Zhao, L.H., Li, M.Y., Wang, X.I., Liu, H.M. (2011). Genetic diversity and genetic relationship of pomegranate (Punica granatum L..) germplasm evaluated by ISSR markers. J Fruit Sci., 01
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