Influence of Storage Condition and Period Time on Fungal Contamination and Mycotoxin in Ruminant Feed

Hozan Jalil Hamasalim; Bahroz Muhammed Saleh  Ahmed; Shagul Jalal  Mohammed

doi:10.35516/jjas.v17i1.65

Authors

Hozan Jalil Hamasalim University of Sulaimanai, Sulaimaniyah, Iraq.
Bahroz Muhammed Saleh Ahmed University of Sulaimanai, Sulaimaniyah, Iraq.
Shagul Jalal Mohammed University of Sulaimanai, Sulaimaniyah, Iraq.

DOI:

https://doi.org/10.35516/jjas.v17i1.65

Keywords:

Feed quality, mycoflora, mycotoxin, ruminant feed

Abstract

Contamination of animal feeds with mycotoxins in livestock farms is a severe matter. This study aimed to evaluate mycotoxigenic fungi, type of toxins, and toxin concentrations and contamination levels of Aflatoxin, Ochratoxin, and T2-toxin in ruminant feed collected from three different sites (a department laboratory, field 1, and field 2) and two periods of time (January and March). Also, four different genera were isolated and diagnosed from the animal feed based on the macroscopic morphology as well as on microscopic characteristics. The genera were Aspergillus spp., Penicillium spp., Fusarium spp., and Rhizopus spp. The result shows significant differences in fungal count among location storages, and among period times, too. Significant differences (P<0.05) also has found for mycotoxin concentration while storage in different locations. Additionally, animal feed collected from different sites and different periods contained lower limits of mycotoxins than the standard limits.

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Author Biographies

Hozan Jalil Hamasalim, University of Sulaimanai, Sulaimaniyah, Iraq.

Department of Animal SciencesCollege of Agricultural Engineering Sciences

Bahroz Muhammed Saleh Ahmed, University of Sulaimanai, Sulaimaniyah, Iraq.

Department of Animal SciencesCollege of Agricultural Engineering Sciences

Shagul Jalal Mohammed, University of Sulaimanai, Sulaimaniyah, Iraq.

Department of Animal SciencesCollege of Agricultural Engineering Sciences

References

Abedullah, N., Mahmood, M. K. and Kouser, S., )2009(. The role of agricultural credit in the growth of livestock sector: A case study of Faisalabad. Pakistan Vet. J., 29(2), pp. 81-84.

Binder, E. M., )2007(. Managing the risk of mycotoxins in modern feed production. Anim. Feed Sci. Technol, 133, pp.149–166.

Bryden, W.L. (2012). Mycotoxin contamination of the feed supply chain: Implications for animal productivity and feed security. Anim. Feed Sci. Technol,173, pp.134–158.

Bryden, W.L. (2004). Mycotoxins and Animal Production: Insidious Problems Associated with Contaminated Feedstuffs. In Proceedings of the International Symposium on Recent Advances in Animal Nutrition, Kuala Lumpur, Malaysia.

Chelkowski, J. (1991). Mycological quality of mixed feeds and ingredients,” in Cereal Grain, Mycotoxins, Fungi and Quality in Drying and Storage, Elsevier, Amsterdam, pp. 217–227.

Čonková E., Laciaková A., Štyriak I., Czerwiecki L. and Wilczyńska G. (2006). Fungal contamination and the levels of mycotoxins (DON and OTA) in cereal samples from Poland and East Slovakia. Czech Journal of Food Sciences. 24: 33-40.

Datsugwai, M. S. S., Ezekiel, B., Audu, Y., Legbo, M. I., Azeh, Y. and Gogo, M. R. (2013). Mycotoxins: Toxigenic Fungal Compounds – A Review. ARPN Journal of Science and Technology, 3(7), pp. 687-692.

Denli, M. and Okan, F., (2006). Efficacy of different adsorbents in reducing the toxin effects of aflatoxin B1 in broiler diets. South Afr. J. Anim. Sci., 36(4), pp. 222-228.

Didwania, N. and Joshi, M. (2013). Mycotoxins: a critical review on occurrence and significance. Int J Pharm Sci, 5(3), pp. 1014-1019.

Dierheimer, G., (1998). Recent advances in the genotoxicity of mycotoxins. Rev Méd Vét,149, pp.585-590.

FAO (Food and Agriculture Organization). (2013). Quality assurance for microbiology in feed analysis laboratories, by R.A. Cowie. Edited by Harinder P.S. Makkar. FAO, Animal Production and Health Manual No. 16. Rome.

Fujimoto, H., (2011). Yeasts and Molds: Mycotoxins: Classification, Occurrence, and Determination. In Encyclopedia of Dairy Sciences, 2nd ed.; Fuquay, J.W., Ed.; Academic Press: San Diego, CA, USA, 2011; pp. 792–800.

Hamasalim, H. J., (2009). The Effect of Different Levels of Feeding on Karadi Lambs Response to Local Iraqi Probiotics. Master’s Thesis, University of Sulaimani, Sulaimani.

Hamasalim, H. J., Hama Khan, K. M. and Sadq, S. M., (2016). Microbiological and mycotoxin risk assessment in ruminant feeding in Sulaimani governorate. Journal of Zankoy Sulaimani – Part A, 18(1), pp.57-64.

International Organization for Standardization (ISO). (2008). Microbiology of food and animal feeding stuffs-Horizontal method for the enumeration of yeasts and moulds-Part 2: Colony count technique in products with water activity less than or equal to 0.95. No. 21527-2.

Kabak, B., Dabson, A.D., and Var, I. (2006). Strategies to prevent mycotoxin contamination of food and animal feed: A review. Critical Reviews in Food Science and Nutrition. 46: 593-619.

Kurata, A. and Ueno, Y., (1984). Toxigenic fungi: Their toxins and health hazard. Odansha-Elseviers Tokyo-Amsterdam-Oxford-New York, pp. 247.

Lacey, J., (1991). Natural occurrence of mycotoxins in growing and conserved forage crops. In: Smith JE, Henderson RE, editors. Mycotoxins and animal foods. Boca Raton, Fla.: CRC Press, pp. 363–397.

Marquardt, R.R., (1996). Effects of molds and their toxins on livestock performance: A western Canadian perspective. Anim. Feed Sci. Technol, 58, pp. 77–89.

Nelson, P.E., Toussoun, T.A., Marasas, W.F.O. (1983). Fusarium Species: An Illustrated Manual for Identification. The Pennsylvania State University Press, University Park, PA.

Okoli, I.C., Omed, A. A., Ogbuewu, I. P., Ekwuagana, I.C., and Ndujihe, G. E. (2007). frequency of mycoflora from commercial poultry feeds and feed raw material in the humid tropical environment of Imo State, Nigeria. Proceeding of 12th annual conference of animal science association Nigeria, pp.115-115.

Oliveira, G.R., Ribeiro, J.M., Fraga, M.E., Cavaglieri, L.R., Direito, G.M., Keller, K.M., Dalcero, A.M., and Rosa, C.A. (2006). Mycobiota in poultry feeds and natural occurrence of aflatoxins, fumonisins and zearalenone in the Rio de Janeiro State, Brazil. Mycopathologia, 162: 355-362.

Oswald, I.P., and Comara, C. (1998). Immunotoxicity of mycotoxins. Rev. Med. Vet., 149, pp. 585–590.

Pitt, J.I., and Hocking, A.D. (1997). Fungi and Food Spoilage. Blackie Academic & Professional, London–NY.

Samson, R.A., Hoekstra, E.S., and Frisvad, J.C. (2004). Introduction to Food and Airborne Fungi. Centraalbureau voor Schimmelcultures, Baarn, Delft.

Seglar, W.J. and Mahanna, W.C. (1995). Mold and Mycotoxin Update. Pioneer Hi-Bred Intl. Sharma R.P. (1993): Immunotoxicity of mycotoxins. Journal of Dairy Science, 76, pp. 892-897.

Steel, R.G.D., Torrie, J.H., and Dickey, D.A.(1996). Principles and procedures of statistics A biometrical approach (3rd ed) McGraw Hill Book Company Inc, New York, USA pp: 334-381.

Sweeney, M.J., Dobson, A.D.W. (1998). Mycotoxin production by Aspergillus, Fusarium, and Penicillium species. Int. J. Food Microbiol, 43, pp. 141–158.

The European Commission for Standardization. (2006). Commission recommendation of 17 August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2, and fumonisins in products intended for animal feeding. Off. J. Eur. Union 2006, 229, 0007–0009.

Yegani, M., Smith, T. K., Lesson, S. and Boermas, H.J. (2006). Effects of feeding grains naturally contaminated with Fusarium mycotoxins on performance and metabolism of broiler breeders. Poult. Sci, 85, pp. 1541- 1549.