Phytochemical Screening and in vitro Anti-urolithiatic Activity of Fruit-seed Extracts of Melia azedarach

Authors

  • Abida Latif Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan
  • Faiza Azhar Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan
  • Muhammad Zohaib Rafay Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan
  • Ahsan Iqbal Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan
  • Iman Anwar Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

DOI:

https://doi.org/10.35516/jjps.v16i1.1074

Keywords:

Melia azedarach, phytochemical analysis, anti-urolithiatic, aggregation assay, nucleation assay

Abstract

Melia azedarach L. has been utilized in traditional systems of medicine for the treatment of various diseases including urolithiasis. The study aimed to perform phytochemical studies and anti-urolithiatic potential of fruit-seed extracts of Melia azedarach. Sequential extraction was performed using chloroform, methanol and water. The extracts of the plant were then subjected to quantitative tests for phytochemical analysis. An anti-urolithiatic activity was investigated via in vitro nucleation and aggregation assay using spectrophotometer. The preliminary phytochemical analysis showed the presence of proteins (31.97 ± 0.56%), lipids (2.95 ± 0.03%) and carbohydrates (64.90 ± 0.56%). The chloroform extract contained the maximum quantity of polyphenols (77.65 ± 0.53 mg/g) and flavonoids (74.71 ± 0.67 mg/g). At a concentration of 5 mg/ml, the chloroform extract exhibited the maximum inhibitory activity in both aggregation and nucleation assay (55.85 ± 1.43%; 56.42 ± 4.49%) respectively. All extracts showed substantial anti-urolithiatic activity by inhibiting the crystallization of calcium oxalate. Due to presence of primary and secondary metabolites, the plant could serve as a source of useful drugs.

Author Biographies

Abida Latif , Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan.

Department of Pharmaceutical Sciences, Akhter Saeed Medical and Dental College, Pakistan.

Faiza Azhar, Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan.

Department of Pharmaceutical Sciences, Akhter Saeed Medical and Dental College, Pakistan.

Muhammad Zohaib Rafay, Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan.

Gulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Pakistan.

Ahsan Iqbal, Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan.

Iman Anwar, Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Pakistan

References

Dharmalingam S.R., Madhappan R., Chidambaram K., et al. Anti-urolithiatic activity of Melia azedarach Linn leaf extract in ethylene glycol-induced urolithiasis in male albino rats. Trop J Pharm Res, 2014; 13 (3): 391-7.

Manasa Reddy J. P.K., Himabindhu J., Ramanjaneyulu K. Evaluation of in Vitro Antiurolithiatic Activity of Mentha Piperita. Int J Pharm Sci Med, 2018; 10 (5): 1236-1237.

Bahuguna Y., Patil K., Jalalpure SJJoTMP. Phytochemical and Pharmacological Investigation of Melia azedarach Leaves for Antiurolithiatic Activity. J Trop Med Plants, 2008; 9 (2): 344-352.

Butterweck V., Khan S.R.J.P. Herbal medicines in the management of urolithiasis: alternative or complementary? Planta Med, 2009; 75 (10): 1095.

Pareta S.K., Patra K.C., Mazumder P.M., Sasmal D.J.J.P.T. Establishing the principle of herbal therapy for antiurolithiatic activity: A review. J Pharmacol Toxicol, 2011; 6 (3): 321-32.

Abo-El-Sooud K., Hashem M., Eleiwa M.M., et al. Antiurolithiatic activity of edible plants and products. Nat Prod, 2012; 8 (4): 168-174.

Khan A., Bashir S., Khan S.R., et al. medicine a. Antiurolithic activity of Origanum vulgare is mediated through multiple pathways. BMC complementary and alternative medicine, 2011; 11 (1): 1-16.

Vyas B., Vyas R., Joshi S., et al. Antiurolithiatic activity of whole-plant hydroalcoholic extract of Pergularia daemia in rats. J Young Pharm, 2011; 3 (1): 36-40.

Abhirama B., ShanmugaSundaram R.J.P.R., Antiurolithic and antioxidant activity of ethanol extract of whole-plant Biophytum sensitivum (Linn.) DC in Ethylene-Glycol-induced urolithiasis in rats. Pharmacognosy Res, 2018; 10 (2): 181.

Sharma D., Paul Y.J.JoAPS. Preliminary and pharmacological profile of Melia azedarach L.: An overview. J App Pharm Sci, 2013; 3 (12): 133-8.

Jafari S., Saeidnia S., Ardekani M.R.S., Hadjiakhoondi A., Khanavi MJTJoB. Micromorphological and preliminary phytochemical studies of Azadirachta indica and Melia azedarach. Turk J Bot, 2013; 37 (4): 690-7.

Farook M., Mohamed H.M., Tariq N.M., et al. Phytochemical screening, Antibacterial and Antioxidant activity of Melia azedarach. Int J Res Anal Rev, 2019; 6 (2): 248-255.

Ahmed M.F., Rao A.S., Ahemad S.R., et al. Phytochemical studies and antioxidant activity of Melia azedarach Linn leaves by DPPH scavenging assay. Int J Pharm App, 2012; 3 (1): 271-276.

Sen A., Batra A.J.I.J.C.P.R. Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. Int J Curr Pharm Res, 2012; 4 (2): 67-73.

Malar T.J., Antonyswamy J., Vijayaraghavan P. et al. In-vitro phytochemical and pharmacological bio-efficacy studies on Azadirachta indica A. Juss and Melia azedarach Linn for anticancer activity. Saudi J Biol Sci, 2020; 27 (2): 682-8.

Shekhawat K.K., Rao D., Batra A.J.Jof et al. Morphological Overview of Medicinal Plant: Melia azedarach Linn. J Func Environ Botany, 2014; 4 (1): 10-21.

Besbes S., Blecker C., Deroanne C., et al. Date seeds: chemical composition and characteristic profiles of the lipid fraction. Food Chem, 2004; 84 (4): 577-584.

Lowry O.H., Rosebrough N.J., Farr A.L., et al. Protein measurement with the Folin phenol reagent. J Biol Chem, 1951; 193 (1): 265-75.

Al-Hooti S., Sidhu S., Gabazard H.J.Jofs., technology. Chemical composition of seeds of date fruit cultivars of United Arab Emirates. J Food Sci, 1998; 35 (1): 44-46.

Slinkard K., Singleton V.L.J.Ajoe., viticulture. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic, 1977; 28 (1): 49-55.

Pavun L., Uskoković-Marković S., Jelikić-Stankov M., et al. Determination of flavonoids and total polyphenol contents in commercial apple juices. Czech J Food Sci, 2018; 36 (3): 233-8.

Hussain K., Ismail Z., Sadikun A., et al. Analysis of proteins, polysaccharides, glycosaponins contents of Piper sarmentosum Roxb. and anti-TB evaluation for bio-enhancing/interaction effects of leaf extracts with Isoniazid (INH). Nat Prod Rad, 2008; 7 (5): 402-408.

Zarin M.A., Tan J.S., Murugan P., et al. Investigation of potential anti-urolithiatic activity from different types of Musa pseudo-stem extracts in inhibition of calcium oxalate crystallization. BMC Complement, 2020; 20 (1): 1-12.

Dilshad R., Batool R. Antibacterial and Antioxidant Potential of Ziziphus jujube, Fagonia Arabica, Mallotus phillipensis and Hemidesmus Indicus. Jordan J Pharm Sci, 2022; 15 (3): 413-27.

Jemal K., Sandeep B.V., Pola S. Phytochemical screening and in vitro antioxidant activity analysis of leaf and callus extracts of Allophylus serratus (ROXB) KURZ. Jordan J Pharm Sci, 2022; 15 (1): 51-69.

Sumathi AJIJPPSS. Evaluation of physicochemical and phytochemical parameters of Melia Azedarach. Leaves (family: meliaceae). Int J Pharm Pharm Sci, 2013; 2 (5): 104.

Ahmed M.F., Rao A.S., Ahmad S.R., et al. Phytochemical studies and antioxidant activity of Melia azedarach Linn leaves by DPPH scavenging assay. Int J Pharm App, 2012; 3 (1): 271-6.

Downloads

Published

2023-03-25

How to Cite

Latif , A. ., Azhar, F. ., Rafay, M. Z., Iqbal, A. ., & Anwar, I. . (2023). Phytochemical Screening and in vitro Anti-urolithiatic Activity of Fruit-seed Extracts of Melia azedarach. Jordan Journal of Pharmaceutical Sciences, 16(1), 137–147. https://doi.org/10.35516/jjps.v16i1.1074

Issue

Section

Articles