Native Medicinal Plants (Moringa oleifera Lam, Brucea javanica (L.) Merr., Eclipta prostrata (L.), Callisia fragrans (Lindl.) Woodson, and Zingiber zerumbet (L.) Smith) in An Giang, Vietnam: A Preliminary Investigation for Rhabdomyosarcoma Treatments using in-vitro RD cell cytotoxicity test
DOI:
https://doi.org/10.35516/jjps.v16i4.1365Keywords:
rhabdomyosarcoma, Moringa oleifera Lam, Brucea javanica (L.) Merr., Eclipta prostrata (L.), Callisia fragrans (Lindl.) Woodson, Zingiber zerumbet (L.) Smith, cytotoxicity;, fractionationAbstract
Cancer, one of the deadliest diseases worldwide, is projected to affect 30.2 million people by 2040. Among the various cancer types, rhabdomyosarcoma (RMS) is a unique tumor primarily impacting the muscular system of children. The current treatment for RMS has limited efficacy and numerous side effects, emphasizing the need for novel therapeutic approaches. This study investigates the potential treatment of the RMS cell line RD using extracts from five folklore-based medicinal plants in An Giang, Vietnam. The plants—Moringa oleifera Lam, Brucea javanica (L.) Merr., Eclipta prostrata (L.), Callisia fragrans (Lindl.) Woodson, and Zingiber zerumbet (L.) Smith—were extracted and fractionated using three solvents: ether, ethanol, and water. These fractions underwent phytochemical screening and cytotoxicity testing on the in-vitro RMS cell line RD. The results indicate that the ether fraction of Eclipta prostrata (L.) and the ether and ethanol fractions of Zingiber zerumbet (L.) Smith exhibit moderate cytotoxic effects on RD cell lines, with IC50 values of 37.08 ± 1.23 µg/mL, 23.15 ± 1.17 µg/mL, and 45.63 ± 2.39 µg/mL, respectively. These findings provide preliminary data for further in-depth research into the anticancer properties of these plants, which are widely grown in the South of Vietnam.
References
Global Cancer Observatory.
Pham, D. T., Saelim, N., and Tiyaboonchai, W., Alpha mangostin loaded crosslinked silk fibroin-based nanoparticles for cancer chemotherapy. Colloids Surf. B. Biointerfaces. 2019; 181, 705–713. DOI: https://doi.org/10.1016/j.colsurfb.2019.06.011
Pham, D. T., Saelim, N., and Tiyaboonchai, W., Paclitaxel loaded EDC-crosslinked fibroin nanoparticles: a potential approach for colon cancer treatment. Drug Deliv. Transl. Res. 2020; 10, 413–424. DOI: https://doi.org/10.1007/s13346-019-00682-7
Kaseb, H., Kuhn, J., and Babiker, H. M., Rhabdomyosarcoma. StatPearls. 2022.
Miwa, S., Yamamoto, N., Hayashi, K., Takeuchi, A., Igarashi, K., and Tsuchiya, H., Recent Advances and Challenges in the Treatment of Rhabdomyosarcoma. Cancers (Basel). 2020; 12. DOI: https://doi.org/10.3390/cancers12071758
Skapek, S. X., Ferrari, A., Gupta, A. A., et al., Rhabdomyosarcoma. Nat. Rev. Dis. Prim. 2019; 5, 1. DOI: https://doi.org/10.1038/s41572-018-0051-2
Dantonello, T. M., Int-Veen, C., Schuck, A., et al., Survival following disease recurrence of primary localized alveolar rhabdomyosarcoma. Pediatr. Blood Cancer. 2013; 60, 1267–1273. DOI: https://doi.org/10.1002/pbc.24488
Malempati, S. and Hawkins, D. S., Rhabdomyosarcoma: review of the Children’s Oncology Group (COG) Soft-Tissue Sarcoma Committee experience and rationale for current COG studies. Pediatr. Blood Cancer. 2012; 59, 5–10. DOI: https://doi.org/10.1002/pbc.24118
Oberlin, O., Rey, A., Lyden, E., et al., Prognostic factors in metastatic rhabdomyosarcomas: results of a pooled analysis from United States and European cooperative groups. J. Clin. Oncol. 2008; 26, 2384–2389. DOI: https://doi.org/10.1200/JCO.2007.14.7207
Weigel, B. J., Lyden, E., Anderson, J. R., et al., Intensive Multiagent Therapy, Including Dose-Compressed Cycles of Ifosfamide/ Etoposide and Vincristine/Doxorubicin/ Cyclophosphamide, Irinotecan, and Radiation, in Patients With High-Risk Rhabdomyosarcoma: A Report From the Children’s Oncology Group. J. Clin. Oncol. 2016; 34, 117–122. DOI: https://doi.org/10.1200/JCO.2015.63.4048
Board, P. P. T. E., Childhood Rhabdomyosarcoma Treatment (PDQ®). PDQ Cancer Inf. Summ. 2022.
Do, T. L. and Nguyen, X. D., Native drugs of Vietnam: which traditional and scientific approaches? J. Ethnopharmacol. 1991; 32, 51–56. DOI: https://doi.org/10.1016/0378-8741(91)90103-K
Nguyen, P. H., Tran, V. D., Pham, D. T., Dao, T. N. P., and Dewey, R. S., Use of and attitudes towards herbal medicine during the COVID-19 pandemic: A cross-sectional study in Vietnam. Eur. J. Integr. Med. 2021; 44, 101328. DOI: https://doi.org/10.1016/j.eujim.2021.101328
Tran, V. D., Pham, D. T., Cao, T. T. N., et al., Perspectives on COVID-19 prevention and treatment using herbal medicine in Vietnam: A cross-sectional study. Ann. Ig. 2021.
Pham, D. T., Thao, N. T. P., Thuy, B. T. P., Tran, V. De, Nguyen, T. Q. C., and Nguyen, N. N. T., Silk fibroin hydrogel containing Sesbania sesban L. extract for rheumatoid arthritis treatment. Drug Deliv. 2022; 29, 882–888. DOI: https://doi.org/10.1080/10717544.2022.2050848
Huynh, D. T. M., Le, M. T., Tran, V. D., and Pham, D. T., Antibacterial hydrogel containing Piper Betel L. extract for acne treatment, an ex vivo investigation. Pharm. Sci. Asia. 2022; 49, 372–380. DOI: https://doi.org/10.29090/psa.2022.04.22.061
Krishnamurthy, P. T., Vardarajalu, A., Wadhwani, A., and Patel, V., Identification and characterization of a potent anticancer fraction from the leaf extracts of Moringa oleifera L. Indian J. Exp. Biol. 2015; 53, 98–103.
Chen, M., Chen, R., Wang, S., et al., Chemical components, pharmacological properties, and nanoparticulate delivery systems of Brucea javanica. Int. J. Nanomedicine. 2013; 8, 85. DOI: https://doi.org/10.2147/IJN.S31636
Timalsina, D. and Devkota, H. P., Eclipta prostrata (L.) L. (Asteraceae): Ethnomedicinal Uses, Chemical Constituents, and Biological Activities. Biomolecules. 2021; 11. DOI: https://doi.org/10.3390/biom11111738
El Sohafy, S. M., Nassra, R. A., D’Urso, G., Piacente, S., and Sallam, S. M., Chemical profiling and biological screening with potential anti-inflammatory activity of Callisia fragrans grown in Egypt. Nat. Prod. Res. 2021; 35, 5521–5524. DOI: https://doi.org/10.1080/14786419.2020.1791113
Zakaria, Z. A., Yob, N. J., Jofrry, S. M., Affandi, M. M. R. M. M., Teh, L. K., and Salleh, M. Z., Zingiber zerumbet (L.) Smith: A Review of Its Ethnomedicinal, Chemical, and Pharmacological Uses. Evid. Based. Complement. Alternat. Med. 2011; 2011. DOI: https://doi.org/10.1155/2011/543216
Paikra, B. K., Dhongade, H. K. J., and Gidwani, B., Phytochemistry and Pharmacology of Moringa oleifera Lam. J. Pharmacopuncture. 2017; 20, 194. DOI: https://doi.org/10.3831/KPI.2017.20.022
Yarmolinsky, L., Zaccai, M., Ben-Shabat, S., and Huleihel, M., Anti-Herpetic Activity of Callissia fragrans and Simmondsia chinensis Leaf Extracts In Vitro. Open Virol. J. 2010; 4, 57. DOI: https://doi.org/10.2174/1874357901004010057
Feng, L., Zhai, Y. Y., Xu, J., et al., A review on traditional uses, phytochemistry and pharmacology of Eclipta prostrata (L.) L. J. Ethnopharmacol. 2019; 245, 112109. DOI: https://doi.org/10.1016/j.jep.2019.112109
Freshney, R. I., Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications: Sixth Edition. Cult. Anim. Cells A Man. Basic Tech. Spec. Appl. Sixth Ed. 2011. DOI: https://doi.org/10.1002/9780470649367
Gerlier, D. and Thomasset, N., Use of MTT colorimetric assay to measure cell activation. J. Immunol. Methods. 1986; 94, 57–63. DOI: https://doi.org/10.1016/0022-1759(86)90215-2
Rani, N. Z. A., Husain, K., and Kumolosasi, E., Moringa genus: A review of phytochemistry and pharmacology. Front. Pharmacol. 2018; 9, 108. DOI: https://doi.org/10.3389/fphar.2018.00108
Aja, P. M., Aja, P. M., Nwachukwu, N., et al., Chemical Constituents of Moringa oleifera Leaves and Seeds from Abakaliki, Nigeria. Am. J. Phytomedicine Clin. Ther. 2014; 2, 310–321.
Tiloke, C., Phulukdaree, A., and Chuturgoon, A. A., The antiproliferative effect of Moringa oleifera crude aqueous leaf extract on cancerous human alveolar epithelial cells. BMC Complement. Altern. Med. 2013; 13. DOI: https://doi.org/10.1186/1472-6882-13-226
Khor, K. Z., Lim, V., Moses, E. J., and Abdul Samad, N., The In Vitro and In Vivo Anticancer Properties of Moringa oleifera. Evid. Based. Complement. Alternat. Med. 2018; 2018. DOI: https://doi.org/10.1155/2018/1071243
Helmi, H. and Susanti, I., Phytochemical tested and in vitro screening antimalaria activity of Belilik Brucea javanica L Merr against Plasmodium falcifarum. J. Biol. Res. 2015; 19, 1–4. DOI: https://doi.org/10.23869/bphjbr.19.1.20131
Su, S. Y., [Treatment of lung cancer with brain metastasis using an intravenous drip of a 10% emulsion of Brucea javanica seminal oil]. Zhong xi yi jie he za zhi = Chinese J. Mod. Dev. Tradit. Med. 1985; 5, 66-67,86-88.
Cuendet, M. and Pezzuto, J. M., Antitumor activity of bruceantin: an old drug with new promise. J. Nat. Prod. 2004; 67, 269–272. DOI: https://doi.org/10.1021/np030304+
Wang, X., Wang, H., Cao, L., et al., Efficacy and Safety of Brucea javanica Oil Emulsion Injection in the Treatment of Gastric Cancer: A Systematic Review and Meta-Analysis. Front. Nutr. 2021; 8. DOI: https://doi.org/10.3389/fnut.2021.784164
Issa, M. E., Berndt, S., Carpentier, G., Pezzuto, J. M., and Cuendet, M., Bruceantin inhibits multiple myeloma cancer stem cell proliferation. Cancer Biol. Ther. 2016; 17, 966–975. DOI: https://doi.org/10.1080/15384047.2016.1210737
Khanna, G. and Krishnan, K., Anticancer-cytotoxic activity of saponins isolated from the leaves of Gymnema sylvestre and Eclipta prostrata on HeLa cells. Int. J. Green Pharm. 2009; 3. DOI: https://doi.org/10.4103/0973-8258.56280
Olennikov, D. N., Ibragimov, T. A., Zilfikarov, I. N., and Chelombit′ko, V. A., Chemical composition of Callisia fragrans juice 1. Phenolic compounds. Chem. Nat. Compd. 2008; 44, 776–777. DOI: https://doi.org/10.1007/s10600-009-9174-8
Olennikov, D., Stolbikova, A., Rokhin, A., Ibragimov, T., and Zilfikarov, I., Chemical composition of Callisia fragrans juice. II. Carbohydrates. Chem. Nat. Compd. - CHEM NAT COMPD. 2010; 46, 273–275. DOI: https://doi.org/10.1007/s10600-010-9585-6
Tian, M., Wu, X., Hong, Y., Wang, H., Deng, G., and Zhou, Y., Comparison of Chemical Composition and Bioactivities of Essential Oils from Fresh and Dry Rhizomes of Zingiber zerumbet (L.) Smith. Biomed Res. Int., (ed. Rinaldo, S.). 2020; 2020, 9641284. DOI: https://doi.org/10.1155/2020/9641284
Dũng, N. X., Chính, T. D., and Leclercq, P. A., Chemical Investigation of the Aerial Parts of Zingiber zerumbet (L.) Sm. from Vietnam. J. Essent. Oil Res. 1995; 7, 153–157. DOI: https://doi.org/10.1080/10412905.1995.9698490
A.B.H, A., Al-Zubairi, A., Nirmala Devi, T., et al., Anticancer Activity of Natural Compound (Zerumbone) Extracted from Zingiber zerumbet in Human HeLa Cervical Cancer Cells. Int. J. Pharmacol. 2008; 4. DOI: https://doi.org/10.3923/ijp.2008.160.168
Sowndhariya, S. S., Ravi, S., Dharani, J. D., and Sripathi, R. S., Chemical Constitution, In-silico Molecular Docking Studies and Antibacterial Activity of Flower Essential Oil of Artabotrys hexapetalus. Jordan J. Pharm. Sci. 2022; 15, 341–354. DOI: https://doi.org/10.35516/jjps.v15i3.408
Osanloo, M., Yousefpoor, Y., Alipanah, H., Ghanbariasad, A., Jalilvand, M., and Amani, A., In-vitro Assessment of Essential Oils as Anticancer Therapeutic Agents: A Systematic Literature Review. Jordan J. Pharm. Sci. 2022; 15, 173–203 DOI: https://doi.org/10.35516/jjps.v15i2.319
