Fertility control impact of the aerial parts Ferula tingitana L. via alteration of hypothalamic-pituitary-gonadal axis responses of female Wistar rats
DOI:
https://doi.org/10.35516/jjps.v15i1.285Keywords:
Contraceptive, Ferula tingitana L., hypoglycemic, sesquiterpene coumarinsAbstract
Ferula tingitana L. has been reported for abortive and\or menstruation inducing properties. However, its contraceptive effect has never been deliberately evaluated. Furthermore, no inclusive chemical profiling of its extract was recorded. Many Ferula species were known for their effects on the oestrogenic rhythm. During our drug discovery from natural sources F. tingitana L. growing in Libya was selected for evaluation of its contraceptive effect. To evaluate the hormonal effects and bioactive molecules of F.tingitana ethanol extract of aerial parts (EtOH) using in vivo experimental model. Adult female albino rats were divided equally into 4 groups (n=6). One group received distilled water for 14days, 2nd ,3rd and 4th groups received orally the tested extract at a daily dose of 100, 200 and 300 mgkg-1 b.wt. for 14 days, respectively. The administration lasted for 14 days (2 weeks) at 9 A.M. Rats body and uterus weight were measured. They fasted overnight and then anaesthetized through a diethylether exposure and blood samples were collected through the ocular puncture. Blood was centrifuged to obtain clear sera for hormonal assay. The serum was subjected by ELISA method for assessment of follicle stimulating hormone (FSH), luteinizing hormone(LH), estradiol(E2)and progesterone (P4) levels. Biochemical estimations of total cholesterol (TC), triglycerlide(TG) and glucose(Glu) level were measured. GC/MS of the lipoidal profile along with HPLC analysis of the phenolic contents were carried out. EtOH and its successive soluble fractions were subjected for chromatographic analysis. The results displayed significant decrease in levels of FSH, LH, E2 and P4 of adult female Wistar rats. Significant decline in biochemical serum level of TC, TG and Glu were observed. Sesquiterpene daucol, linolenic acid, caffeic acid and hesperidin were the main identified phytoconstituents. CC of EtOH afforded 5 compounds were identified as β-sitosterol 1, colladonin 2, scopoletin 3, caffeic acid 4, 1-(3,4-dihydroxycinnamoyl) cyclopentane-2`,3`-diol 5. It was concluded that sesquiterpene coumarins as the significant phytoconstituents of EtOH may revealing adverse effect on the menstruation, ovulation of follicles and consequently may impair fertility. EtOH evidenced a hypoglycemic and hypocholesterolemic effects.
References
(1) Birdsall N, Kelley AC and Steven W Sinding, eds. (2001). Population Matters: Demographic Change, Economic Growth, and Poverty in the Developing World. Oxford: Oxford University Press.
(2) Bloom DE and Canning D, "Global Demographic Change: Dimensions and Economic Significance", Population and Development Review vol. 33 (supplement), Population Council, New York, 2008; 17-51
(3) United Nations, Department of Economic and Social Affairs, Population Division,2009. World Population Prospects: The 2008 Revision Population Database. Available from http:// esa.un.org/unpp (accessed 14 July 2010).
(4) Alipour Z, Taheri P, Samadi N. Chemical composition and antibacterial activity of the essential oils from flower, leaf and stem of Ferula cupularis growing wild in Iran. Pharm Biol, 2015;53: 483-487.
(5) Mohammadhosseini M, Mahdavi B, Shahnama M. Chemical composition of essential oils from aerial parts of Ferula gummosa (Apiaceae) in Jajarm Region, Iran using traditional hydrodistillation and solvent-free microwave extraction methods: A comparative approach. J Essent Oil-Bear Pl, 2015;18: 1321-1328.
(6) Mohammadhosseini M, Venditti A, Sarker SD, Nahar L, Akbarzadeh A. The genus Ferula: Ethnobotany, phytochemistry and bioactivities – A review. Ind Crops Prod. 2019; 129: 350-394.
(7) Singh, M., Agnihotri, A., Garg, S., Agarwal, K, S., Gupta, N.D., Keshri, G., Kamboj, P. V.,1989. Antifertility and Hormonal Properties of Certain Carotane Sesquiterpenes of Ferula jaeschkeana. Planta Med., 54,492-494.
(8) Prakash, A., Pathak, S., Shaiv, A., Mathur, R., 1994. oestrogenic rhythm induced by a single administration of the extract of Ferula jaeschkeana to immature rats. Phytother Res., 8, 28-32.
(9) Miski M, Ulubelen A, Mabry TJ, Watson WH, Vickovic I, Holub M, A new sesquiterpene ester from Ferula tingitana.Tetrahedron 1984; 40:5197-5201.
(10) Miski M, Mabry T J. New daucane esters from Ferula tingitana. J Nat Prod, 1986;49:657-660.
(11) Elghwaji W, El-Sayed AM, El-Deeb KS, ElSayed AM. Chemical Composition, Antimicrobial and Antitumor Potentiality of Essential Oil of Ferula tingitana L. Apiaceae Grow in Libya. Pharmacogn Mag. 2017;13 (Suppl3):S446- S451.doi:10.4103/pm.pm_323_15
(12) Vogel A. a textbook of practical organic chemistry, 3rd, english language book society and longman group ltd., London 1975.
(13) Geissman TA, Ollis W, Domínguez X, Deverall B, Dunn A, Arditti J, Guzmán M, Romero R, Rivera G, Mora F. The chemistry of flavonoid compounds, centro regional de ayuda técnica, México, DF (méxico) 1962.
(14) Ivanova V, Stefova M, Chinici F. Determination of the polyphenol contents in Macedonian grapes and vines by standardized spectrophotometric methods. J Serb Chem Soc. 2010;72: 45-49.
(15) Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal., 2002;10: 178–182.
(16) Goupy P, Hugues M, Boivin P, Amiot J. Antioxidant composition and activity of barley (Hordeum vulgare) and malt extract and of isolated phenolic compounds. J Sci Food Agr. 1999;79: 1625-1634.
(17) Mattila P, Astole J, Kumpulainen J. Determination of flavonoids in plant material by HPLC with diode-array and electro-arraydetections J Sci Food Agric., 2000; 48: 5834-5841
(18) Sakamoto S, Putalun W, Vimolmangkang S, Phoolcharoen W, Shoyama Y, Tanaka H, & Morimoto S. Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites. J Nat Med, 2018;72(1): 32–42.
doi:10.1007/s11418-017-1144-z
(19) Uotila M, Ruoslahti E, Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J. Immunol Methods 1981;42: 11-15.
(20) King E J, Garner, R J. The Colorimetric Determination of Glucose. J clin pathol. 19471;(1);30–33. doi:10.1136/jcp.1.1.30
(21) Henery RJ, Cannon DC, Winkelman JW. Clinical chemistry principles anfd techiness, Bew York;Harper and Row; 1997;.1440.
(22) Fossati P, Principle l. Estimation of the concentration of triglyceride in plasma and liver. Clinical Chemistry, 1982;28: 2077-2081.
(23) Sahebkar A, Iranshahi M Volatile constituents of the genus Ferula (Apiaceae): A review. J Essent Oil Bear Pl., 2011;14:504–531.
(24) Ku C-M, Lin J-Y. Anti-inflammatory effects of 27 selected terpenoid compounds tested through modulating TH1/TH2 cytokine secretion profiles using murine primary splenocytes. Food Chem, 2013; 141: 1104-1113.
(25) Hazar A, Raed A, Nidal J, Motasem M. Evaluation of phytochemical and pharmacological activities of Taraxacum syriacum and Alchemilla arvensis Jordan Journal of Pharmaceutical Sciences, 2021; 14 (4); 457-473.
(26) Lefahal M, Zaabat N, Ayad R, Makhloufi EH, Djarri L, Benahmed M, Laouer H, Nieto G, Akkal S. In Vitro Assessment of Total Phenolic and Flavonoid Contents, Antioxidant and Photoprotective Activities of Crude Methanolic Extract of Aerial Parts of Capnophyllum peregrinum (L.) Lange (Apiaceae) Growing in Algeria. Medicines (Basel), 2018;5(2): 26.
(27) Goad LJ, Akihisa T. Analysis of sterol. Published by Blackie Academic & Professional, London. 1997.
(28) Abd EL‐Razek M H, Wu Y C, Chang F R. Sesquiterpene coumarins from Ferula foetida. J Chin Chem Soc-TAIP. 2007; 54: 235-238.
(29) Özek G, Özek T, Işcan G, Başer K H C, Duran A. Hamzaoglu EComposition and antimicrobial activity of the oils of Ferula szowitsiana dc. from turkey. J Essent oil Res.2008; 20: 186-190.
(30) Iqra H K, Arshad J.Identification of Biologically Important Compounds In Neem Leaves Through GC-MS Analysis Jordan Journal of Pharmaceutical Sciences 2021;14(3):359-367.
(31) Jalili-Nik M, Soukhtanloo, M., Javanshir, S., Yazdi, A., Esmaeilizadeh, M., Jafarian, A., Ghorbani, A., 2019. Effects of ethanolic extract of Ferula gummosa oleo-resin in a rat model of streptozotocin-induced diabetes. Res Pharm Sci, 14 (2); 138-145.
(32) Kumar, D., Kumar, A., Prakash, O., 2012. Potential antifertility agents from plants: a comprehensive review. J Ethnopharmacol, 140, 1-32.
(33) Yusufoglu HS, Soliman GA, Abdel-Rahman RF, et al. Antihyperglycemic and antihyperlipidemic effects of Ferula duranii in experimental type 2 diabetic rats. Int J Pharmacol. 2015; 11: 532-541.
(34) Singh R, Kakar S, Shah M, Jain R Some Medicinal Plants with Anti-Fertility Potential: A Current Status; 2018. doi: 10.4103/2278-960X.194512
(35) Ogbuewu I P, Unamba-Oparah I C, Odoemenam V U, Etuk I F, Okoli I C. The potentiality of medicinal plants as the source of new contraceptive principles in males N Am J Med Sci., 2011;3(6): 255–263.
(36) Gopalkrishnan B, Shimpi SN. Antifertility effect of Madhuca latifolia (roxb.) Macbride seed extract. Int J Appl Biol Pharm Tech. 2011;2(4):49-53.
(37) Zade V, Wikhe M, Dabhadkar D, Dawada S, Patil U. Antifertility efficacy of Cannabis sativa leaves on female albino rats. Int J Sci Invent Today.2013; 2(2):107-117.
(38) Nadkarni KM. Indian Materia Medica, Vol. I. Popular Prakashan Pvt. Ltd, Bombay, India. 1976.
(39) Hashimoto I, Isomoto N, Eto M, Kawaminami M, Sunazuka C, Ueki N. preovulatory secretion of progesterone, luteinizing hormone, and prolactin in 4-day and 5-day cycling rats. Biology of Reproduction. 1987;36: 599-605.
(40) AL-Shemary N N A, Mousa S A, Muslim Z Z. Histological and hormonal study about the effect of aqueous extract of ocimum gratissmum on female reproductive system in albino mice. J Pharmaceut Sci Res, 2018;10: 765-767.
(41) Maltaris T, Koelbl H, Seufert R, Kiesewetter F, Beckmann M W, Mueller A, Dittrich R. Gonadal damage, and options for fertility preservation in female and male cancer survivors. Asian J Androl 2006; 8:515-533.
(42) Kalita J, Chakrabarty A, Tanti B. Assessment of Antifertility activity of some traditionally used plants by different ethnic communities in three districts of Assam, India. J. Herbal Med. Toxicol. 2011;5: 65–72.
(43) Singh M, Agnihotri A, Garg S, Agarwal K S, Gupta ND, Keshri G, Kamboj PV. Antifertility and Hormonal Properties of Certain Carotane Sesquiterpenes of Ferula jaeschkeana. Planta Med., 1989;54:492-494.
(44) Chen B, Teranishi R, Kawazoe K, Takaishi Y, Honda G, Itoh M, Takeda Y, Kodzhimatov OK. Sesquiterpenoids from Ferula kuhistanica. Phytochemistry 2000; 54: 717-722.
(45) Rincon-Cervera M A, Valenzuela R, Hernandez-Rodas MC, Barrera C, Espinosa E, Marambio, M, Valenzuela A. Vegetable oils rich in alpha linolenic acid increment hepatic n-3 LCPUFA, modulating the fatty acid metabolism and antioxidant response in rats. Prostaglandins Leukot Essent Fatty Acids 2016; 111: 25-35.
