Preparation and Characterization of Hydrogel Beads for Controlled Release of Amoxicillin
DOI:
https://doi.org/10.35516/jjps.v15i4.675Keywords:
Controlled drug delivery, Sodium alginate, Hydrogel beads, Poloxamer 407, AmoxicillinAbstract
Amoxicillin trihydrate-loaded hydrogel beads were prepared and characterized as a controlled drug delivery system to improve patient compliance. An ionotropic gelation process was used to prepare the hydrogel beads using calcium chloride (CaCl2) as a crosslinking agent. The effects of CaCl2, sodium alginate, poloxamer 407 (PL) concentration, and preparation temperature were investigated. Spherical hydrogel beads were obtained with high encapsulation efficiency (85.74±1.09) %. FTIR analyses confirmed the compatibility of amoxicillin with the used excipients. In vitro swelling and cumulative drug release studies were performed over 24 hours in HCl medium, pH 1.2. Prepara-tion temperature was found to influence both the index of beads swelling (SI) and the cumulative release of amoxicil-lin. The study demonstrated the capability of PL to enhance the cumulative release of amoxicillin, correlating with swelling behavior. The proposed hydrogel beads have the potential as a promising drug delivery system for controlled release of amoxicillin, over 24 hours, and thus reduced dosing frequency.
References
Dey, S. K. et al. Floating mucoadhesive alginate beads of amoxicillin trihydrate: A facile approach for H. pylori eradication. International Journal of Biological Macromolecules. 2016; 89: 622–631.
Al-Degs, Y. S., El-Sheikh, A. H. & Harb, D. M. Accurate Quantification of Amoxicillin in Different Drug Formulations using Advanced Chemometric Methods. Jordan Journal of Pharmaceutical Sciences. 2020; 13: 19-27.
Cammarota, G., Sanguinetti, M., Gallo, A. & Posteraro, B. Review article: biofilm formation by Helicobacter pylori as a target for eradication of resistant infection. Alimentary Pharmacology & Therapeutics. 2012; 36: 222–230.
Alfaqeer, R. H., Albakain, R., Rasheed, M. & Makahleh, A. Development of Novel HPLC Method for Analysing Drugs Used in H-Pylori Treatment. Jordan Journal of Pharmaceutical Sciences. 2021; 14: 473-486.
Thambavita, D. et al. Biowaiver Monograph for Immediate-Release Solid Oral Dosage Forms: Amoxicillin Trihydrate. J Pharm Sci. 2017; 106: 2930–2945.
Llor, C. et al. A study of adherence to antibiotic treatment in ambulatory respiratory infections. International Journal of Infectious Diseases. 2013; 17: e168–e172.
Michael E. Aulton & Kevin M.G. Taylor. Aulton’s pharmaceutics: the design and manufacture of medicines; Churchill Livingstone: Elsevier, New York. 2013, p 552.
El-Mahrouk, G. M., Aboul-Einien, M. H. & Makhlouf, A. I. Design, Optimization, and Evaluation of a Novel Metronidazole-Loaded Gastro-Retentive pH-Sensitive Hydrogel. AAPS PharmSciTech. 2016; 17: 1285–1297.
Kalshetti, P. P., Rajendra, V. B., Dixit, D. N. & Parekh, P. P. Hydrogels as a Drug Delivery System and Applications: A REVIEW. International Journal of Pharmacy and Pharmaceutical Science. 2012; 4:1-7.
Altinisik, A. & Yurdakoc, K. Chitosan/poly (vinyl alcohol) hydrogels for amoxicillin release. Polym. Bull. 2014; 71: 759–774.
Narkar, M., Sher, P. & Pawar, A. Stomach-Specific Controlled Release Gellan Beads of Acid-Soluble Drug Prepared by Ionotropic Gelation Method. AAPS PharmSciTech 2010; 11: 267-277.
Motwani, S. K. et al. Chitosan-sodium alginate nanoparticles as submicroscopic reservoirs for ocular delivery: formulation, optimisation and in vitro characterisation. Eur J Pharm Biopharm. 2008; 68: 513–525.
Giri, T. K. et al. Alginate based hydrogel as a potential biopolymeric carrier for drug delivery and cell delivery systems: present status and applications. Curr Drug Deliv 2012; 9: 539–555.
Parhi, R. & Suresh, P. Alginate-Poloxamer Beads for Controlled Release of Metoprolol Succinate. Turkish Journal of Pharmaceutical Science. 2015; 12: 59-66.
López-Cacho, J. M., González-R, P. L., Talero, B., Rabasco, A. M. & González-Rodríguez, M. L. Robust Optimization of Alginate-Carbopol 940 Bead Formulations. The Scientific World Journal. 2012; 4: 1–15.
Angadi, S. C., Manjeshwar, L. S. & Aminabhavi, T. M. Novel composite blend microbeads of sodium alginate coated with chitosan for controlled release of amoxicillin. International Journal of Biological Macromolecules. 2012; 51: 45–55.
Moebus, K., Siepmann, J. & Bodmeier, R. Alginate–poloxamer microparticles for controlled drug delivery to mucosal tissue. European Journal of Pharmaceutics and Biopharmaceutics. 2009; 72: 42–53.
Giuliano, E., Paolino, D., Fresta, M. & Cosco, D. Mucosal Applications of Poloxamer 407-Based Hydrogels: An Overview. Pharmaceutics. 2018; 10 (3): 159.
Mansuri, S., Kesharwani, P., Jain, K., Tekade, R. K. & Jain, N. K. Mucoadhesion: A promising approach in drug delivery system. Reactive and Functional Polymers. 2016; 100: 151–172.
Swed, A. et al. Sustained release of TGF-β1 from biodegradable microparticles prepared by a new green process in CO2 medium. International Journal of Pharmaceutics. 2015; 493: 357–365.
Swed, A., Cordonnier, T., Fleury, F. & Boury, F. Protein Encapsulation into PLGA Nanoparticles by a Novel Phase Separation Method Using Non-Toxic Solvents. Journal of Nanomedicine & Nanotechnology. 2014; 5: 1-8.
Khoder, M., Tsapis, N., Domergue-Dupont, V., Gueutin, C. & Fattal, E. Removal of residual colonic ciprofloxacin in the rat by activated charcoal entrapped within zinc-pectinate beads. European Journal of Pharmaceutical Sciences. 2010; 41: 281–288.
Dalaty, A. A., Karam, A., Najlah, M., Alany, R. G. & Khoder, M. Effect of non-cross-linked calcium on characteristics, swelling behaviour, drug release and mucoadhesiveness of calcium alginate beads. Carbohydrate Polymers. 2016; 140: 163–170.
Nayak, A. K. & Pal, D. Development of pH-sensitive tamarind seed polysaccharide–alginate composite beads for controlled diclofenac sodium delivery using response surface methodology. International Journal of Biological Macromolecules. 2011; 49: 784–793.
Shargel, L. & Yu, A. B. C. Applied biopharmaceutics & pharmacokinetics; Mc Graw Hill Education: New York. 2016, p 435.
Smrdel, P. The Influence of Selected Parameters on the Size and Shape of Alginate Beads Prepared by Ionotropic Gelation. Scientia Pharmaceutica. 2008; 76: 77–89.
Arora, S. & Budhiraja, R. D. Chitosan-alginate microcapsules of amoxicillin for gastric stability and mucoadhesion. J Adv Pharm Technol Res. 2012; 3: 68–74.
Jeong, C., Kim, S., Lee, C., Cho, S. & Kim, S.-B. Changes in the Physical Properties of Calcium Alginate Gel Beads under a Wide Range of Gelation Temperature Conditions. Foods. 2020; 9 (2):180.
Morakul, B., Suksiriworapong, J., TraidejChomnawang, M., Langguth, P. & BurapacheepJunyaprasert, V. Dissolution enhancement and in vitro performance of clarithromycin nanocrystals produced by precipitation–lyophilization–homogenization. European Journal of Pharmaceutics and Biopharmaceutics. 2014; 88: 886–896.
Huang, S.-L. & Lin, Y.-S. The Size Stability of Alginate Beads by Different Ionic Crosslinkers. Advances in Materials Science and Engineering. 2017; 1: 1-7.
Florence, A. T. & Attwood, D. Physicochemical principles of pharmacy; pharmaceutical press: London. 2007, p 297.
Lotfipour, F., Mirzaeei, S. & Maghsoodi, M. Evaluation of the effect of CaCl2 and alginate concentrations and hardening time on the characteristics of Lactobacillus acidophilus loaded alginate beads using response surface analysis. Adv Pharm Bull. 2012; 2: 71–78.
Pasparakis, G. & Bouropoulos, N. Swelling studies and in vitro release of verapamil from calcium alginate and calcium alginate-chitosan beads. Int J Pharm. 2006; 323: 34–42.
Morris, N., Razak, F., Kennedy, J. & Murphy, A. Development of Amoxicillin loaded microspheres for anti-Helicobacter pylori infection using Ionic Gelation method. 2017; 2: 56-67.
Croy, S. R. & Kwon, G. S. The effects of Pluronic block copolymers on the aggregation state of nystatin. J Control Release. 2004; 95: 161–171.
Martín-Villena, M. J. et al. Novel microparticulate systems for the vaginal delivery of nystatin: development and characterization. Carbohydr Polym. 2013; 94: 1–11.
