التركيب الكيميائي والتقييم البيولوجي للبروبوليس الجزائري من ست مناطق مختلفة
DOI:
https://doi.org/10.35516/jjps.v16i2.1319الكلمات المفتاحية:
البروبوليس، مضادات الأكسدة، مضادات الميكروبات، عديد الفينولات، الجزائرالملخص
يعتبر البروبوليس راتينجًا طبيعيًا ينتجه النحل ولا يزال يستخدم في الطب الشعبي. تم فحص ست عينات بروبوليس من نحل العسل الغربي (P1-P6) تم جمعها من مناطق مختلفة في الجزائر لمعرفة محتوياتها وأنشطتها البيولوجية. أظهرت النتائج المتحصل عليها أن البروبوليس P1 أظهر أعلى نسبة من الفينولات الكلية (210.93 مجم من GAE / جم بروبوليس)، وفلافونويد كلي (34.33 مجم QE / جم بروبوليس)، والتانين (23.36 مجم / جم بروبوليس). بالنسبة للأنشطة المضادة للأكسدة، أظهر P1 نشاطًا قويًا في إزالة الجذور الحرة بقيم EC50 تبلغ 0.055 و 0.0306 و 0.109 و 0.071 مجم / مل على التوالي لمقايسات DPPH و ABTS و FRAP و phosphomolybdenum. من ناحية أخرى، أظهرت جميع أنواع البروبوليس نشاطًا مضادًا للبكتيريا ضد بكتيريا G + ve (S. aureus) مع أنشطة أعلى قليلاً مرتبطة بـعينات P1 و P5 (9.83 و 10.92 مم، على التوالي). أظهر P5 أدنى MIC وMBC ضد S. aureus بقيم 62.5 و 125 ميكروجرام / مل على التوالي. علاوة على ذلك ، كان لجميع عينات البروبوليس أنشطة متوسطة إلى منخفضة كمضادات للميكروبات ضد) C. albicans الخميرة) مع أنشطة متوسطة لـعينات P1 و P6 (8.50 و13.33 مم، على التوالي). كما أدى التنميط الكيميائي لعينات البروبوليس الأكثر نشاطًا بيولوجيًا (P1 و P4 و P5) بإستخدام تحليل بصمات الأصابع (HPLC-fingerprint) بشكل أساسي إلى إكتشاف الأحماض الفينولية والفلافونويدات بنسب متغيرة.
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