تقييم النشاط المضاد للالتهابات ومضادات الأكسدة وتحليل الالتحام الجزيئي لمستخلص أوراقنبات الروبوس إيديوس
DOI:
https://doi.org/10.35516/jjps.v17i1.1808الكلمات المفتاحية:
روبوس ايديوس ل.، ورقة، HPLC، الالتحام الجزيئي، النشاط المضاد للأكسدة، النشاط المضاد للالتهابات، الارتباطالملخص
هدفت الدراسة إلى تحديد المركبات الأكثر وفرة في مستخلص أوراق التوت عن طريق HPLC، وإجراء دراسات نظرية وعملية لتقييم الأنشطة المضادة للأكسدة والمضادة للالتهابات في السيليكو وفي المختبر وفي الجسم الحي، والتحقيق في العلاقة بين الأنشطة المضادة للأكسدة والمضادة للالتهابات. تم تحديد كمية البوليفينول باستخدام HPLC، وتم إجراء الإرساء الجزيئي باستخدام AutoDockTools 1.5.6، وتم تحديد النشاط المضاد للأكسدة باستخدام طريقة قياس الجهد، ودراسة النشاط المضاد للالتهابات باستخدام طريقة الوذمة الكاراجينية. وُجد أن المستخلص غني بمادة الإبيكاتشين (0.417%) و(+)-كاتشين (0.501%) والإيلاجوتانين (0.401%). كانت الطاقة الحرة لـ (+) -كاتيشين وإبيكاتشين 8.40- و 7.20- على التوالي للمواقع النشطة لأنزيمات الأكسدة الحلقية-2 (COX-2)، و 6.60- و 7.11- لأنزيمات أوكسيديز فوسفات نيكوتيناميد الأدينين أدينين ثنائي النوكليوتيد (NADPH oxidase). كان النشاط المضاد للأكسدة لمستخلص أوراق التوت أعلى بنسبة 1.43% و1.04% و10.62% من مستخلص أوراق الشاي الأخضر لجرعات 4.00 و2.00 و0.20 ملغم/ملل على التوالي. أظهر العلاج بمستخلص أوراق التوت بجرعة 13.0 ملغم/كغم انخفاضًا ملحوظًا في الوذمة بعد 1 و2 و3 ساعات بنسبة 38.8% و41.8% و48.8% على التوالي مقارنة بالمجموعة الضابطة. كشفت الدراسة عن وجود تطابق بين النتائج التجريبية والنظرية في تقييم الأنشطة المضادة للأكسدة والمضادة للالتهابات. أكد تحليل الارتباط اعتماد النشاط المضاد للالتهابات على النشاط المضاد للأكسدة.
المراجع
Biswas SK. Does the Interdependence between Oxidative Stress and Inflammation Explain the Antioxidant Paradox? Oxidative Med Cell Longev. 2016; 2016:1-9. Doi: https://doi.org/10.1155/2016/5698931 DOI: https://doi.org/10.1155/2016/5698931
Maslov OY., Kolisnyk SV., Komissarenko NA., Kostina TA. Development and validation potentiometric method for determination of antioxidant activity of epigallocatechin-3-O-gallate. Pharmacologyonline. 2021; 2:35-42.
Doi: https://doi.org/10.5281/zenodo.7813098
Maslov OY., Komisarenko MA., Golik MY., Kolisnyk SV., Altukhov AA., Baiurka SV., Karpushina SA., Tkachenko O., Iuliia K. Study of total antioxidant capacity of red raspberry (Rubus idaeous L.) shoots. Vitae. 2023; 30(1).
Doi: https://doi.org/10.17533/udea.vitae.v30n1a351486 DOI: https://doi.org/10.17533/udea.vitae.v30n1a351486
Li J., Lan T., Zhang C., Zeng C., Hou J., Yang Z., Zhang M., Liu J., Liu B. Reciprocal activation between IL-6/STAT3 and NOX4/Akt signalings promotes proliferation and survival of non-small cell lung cancer cells. Oncotarget. 2015; 6(2):1031-48. Doi: https://doi.org/10.18632/oncotarget.2671 DOI: https://doi.org/10.18632/oncotarget.2671
Fan Y., Mao R., Yang J. NF-κB and STAT3 signaling pathways collaboratively link inflammation to cancer. Protein Amp Cell. 2013; 4(3):176-85. Doi: https://doi.org/10.1007/s13238-013-2084-3 DOI: https://doi.org/10.1007/s13238-013-2084-3
Ismail WH., Abusara OH., Ikhmais B., Abul-Futouh H., Sunoqrot S., Ibrahim AI. Design, Synthesis, and Biological Activity of Coniferyl Aldehyde Derivatives as Potential Anticancer and Antioxidant Agents. Jordan j. pharm. Sci. 16(2):368-80.
Doi: https://doi.org/10.35516/jjps.v16i2.1463 DOI: https://doi.org/10.35516/jjps.v16i2.1463
Yarza R., Vela S., Solas M., Ramirez MJ. c-Jun N-terminal Kinase (JNK) Signaling as a Therapeutic Target for Alzheimer’s Disease. Front Pharmacol. 2016; 6. Doi: https://doi.org/10.3389/fphar.2015.00321 DOI: https://doi.org/10.3389/fphar.2015.00321
Gudej J. and Tomczyk M. Determination of Flavonoids, Tannins and Ellagic acid in leaves from Rubus L. species. Arc Pharm Res. 2004; 27(11):1114-1119. Doi: https://doi.org/10.1007/BF02975114 DOI: https://doi.org/10.1007/BF02975114
Kim M., Sutton K., Harris G. Raspberries and Related Fruits, second ed.; Academic Press, Oxford, UK 2016.
Doi: https://doi.org/10.1016/B978-0-12-384947-2.00586-9 DOI: https://doi.org/10.1016/B978-0-12-384947-2.00586-9
Jemal K., Sandeep BV., Pola S. Phytochemical screening and in vitro antioxidant activity analysis of leaf and callus extracts of Allophylus serratus (ROXB) KURZ. Jordan j. pharm. sci. 2022; 15(1):51-69. Doi: https://doi.org/10.35516/jjps.v15i1.291 DOI: https://doi.org/10.35516/jjps.v15i1.291
Durgo K., Belščak-Cvitanović A., Stančić A., Franekić J., Komes D. The Bioactive Potential of Red Raspberry (Rubus idaeus L.) Leaves in Exhibiting Cytotoxic and Cytoprotective Activity on Human Laryngeal Carcinoma and Colon Adenocarcinoma. J Med Food. 2012; 15(3):258-268.
Doi: https://doi.org/10.1089/jmf.2011.0087 DOI: https://doi.org/10.1089/jmf.2011.0087
Polischuk IM., Koshovyi OM., Osolodchenko TP., Komissarenko MA. The study of phenolic compounds and the antimicrobial action of the alcoholic extract from the cake of the red raspberry fruit. Visnik Farm. 2018; 3(95):30-3. Doi: https://doi.org/10.24959/nphj.18.2220 DOI: https://doi.org/10.24959/nphj.18.2220
Polishchuk IM., Komisarenko MA., Golik MY., Upyr TV. The study of saponins of the raspberry cake alcoholic extract by HPLC. Visnik Farm. 2018; 4(96):24-7. Doi: https://doi.org/10.24959/nphj.18.2230 DOI: https://doi.org/10.24959/nphj.18.2230
Komisarenko MA., Polischuk IM., Upyr TV., Saidov NB. Study of Amino acid composition and immunomodulatory activity of Rubus idaeus alcoholic extract. RES J PHARM TECHNOL. 2021; 14(3):1329-32.
Doi: https://doi.org/10.5958/0974-360x.2021.00236.5 DOI: https://doi.org/10.5958/0974-360X.2021.00236.5
Ghorbanzadeh B., Mansouri M., Hemmati A., Naghizadeh B., Mard S., Rezaie A. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol. 2015; 47(3):292.
Doi: https://doi.org/10.4103/0253-7613.157127 DOI: https://doi.org/10.4103/0253-7613.157127
Yang DJ, Liu SC, Chen YC, Hsu SH, Chang YP, Lin JT. Three Pathways Assess Anti-Inflammatory Response of Epicatechin with Lipopolysaccharide-Mediated Macrophage RAW264.7 Cells. J Food Biochem. 2015; 39(3):334-43. Doi: https://doi.org/10.1111/jfbc.12134 DOI: https://doi.org/10.1111/jfbc.12134
Maslov O., Komisarenko M., Ponomarenko S., Horopashna D., Osolodchenko T., Kolisnyk S., Derymedvid L., Shovkova Z., Akhmedov E. Investigation the influence of biologically active compounds on the antioxidant, antibacterial and anti-inflammatory activities of red raspberry (Rubus idaeous l.) leaf extract. Curr Issues Pharm Med Sci. 2022. Doi: https://doi.org/10.2478/cipms-2022-0040 DOI: https://doi.org/10.2478/cipms-2022-0040
Maslov OY., Kolisnyk SV., Komisarenko MA., Kolisnyk OV., Ponomarenko SV. Antioxidant activity of green tea leaves (Camellia sinensis L.) liquid extracts. Pharmacologyonline. 2021; (3):291-8.
Doi: https://doi.org/10.5281/zenodo.7813115
Khoddami A., Wilkes M., Roberts T. Techniques for Analysis of Plant Phenolic Compounds. Molecules. 2013; 18(2):2328-75.
Doi: https://doi.org/10.3390/molecules18022328 DOI: https://doi.org/10.3390/molecules18022328
Morris GM., Huey R., Olson AJ. Using AutoDock for Ligand‐Receptor Docking. Curr Protoc Bioinform. 2008; 24(1).
Doi: https://doi.org/10.1002/0471250953.bi0814s24 DOI: https://doi.org/10.1002/0471250953.bi0814s24
RCSB PDB: Homepage. RCSB PDB: Homepage. Access: https://www.rcsb.org/.
PubChem. Access: https://pubchem.ncbi.nlm.nih.gov/
CASTp 3.0: Computed Atlas of Surface Topography of proteins. Access: http://sts.bioe.uic.edu/castp/index.html?201l
Maslov O., Kolisnyk S., Komisarenko M., Golik M. Study of total antioxidant activity of green tea leaves (Camellia sinensis L.). Herba Pol. 2022; 68(1):1-9. Doi: https://doi.org/10.2478/hepo-2022-0003 DOI: https://doi.org/10.2478/hepo-2022-0003
Stefanova OV. Preclinical studies of medicinal products: method.guidance. Kiev: Vidavnichy dim “Avitsena”; 2001:528.
Doi: https://doi.org/10.5281/zenodo.8139960
Maslov OY., Kolisnyk SV., Komisarenko MA., Altukhov AA., Dynnyk KV., Stepanenko VI. Study and evaluation antioxidant activity of dietary supplements with green tea extract. Curr Issues Pharm Med. 2021; 14(2):215-9.
Doi: https://doi.org/10.14739/2409-2932.2021.2.233306 DOI: https://doi.org/10.14739/2409-2932.2021.2.233306
Salminen JP. Recent Advances in Polyphenol Research. Chichester, UK: John Wiley & Sons, Ltd; 2014. The Chemistry and Chemical Ecology of Ellagitannins in Plant-Insect Interactions: From Underestimated Molecules to Bioactive Plant Constituents; 83-113. Doi: https://doi.org/10.1002/9781118329634.ch4 DOI: https://doi.org/10.1002/9781118329634.ch4
Kashchenko NI., Olennikov DN., Chirikova NK. Metabolites of Siberian Raspberries: LC-MS Profile, Seasonal Variation, Antioxidant Activity and, Thermal Stability of Rubus matsumuranus Phenolome. Plants. 10(11):2317.
Doi: https://doi.org/10.3390/plants10112317 DOI: https://doi.org/10.3390/plants10112317
Salminen JP., Roslin T., Karonen M., Sinkkonen J., Pihlaja K., Pulkkinen P. Seasonal Variation in the Content of Hydrolyzable Tannins, Flavonoid Glycosides, and Proanthocyanidins in Oak Leaves. J Chem Ecol. 2004; 30(9):1693-711.
Doi: https://doi.org/10.1023/b:joec.0000042396.40756.b7 DOI: https://doi.org/10.1023/B:JOEC.0000042396.40756.b7
Lopes AJ., Vasconcelos CC., Garcia JB., Pinheiro MS., Pereira FA., Camelo DD., Morais SV., Freitas JR., Rocha CQ., Ribeiro MN., Cartágenes MD. Anti-Inflammatory and Antioxidant Activity of Pollen Extract Collected by Scaptotrigona affinis postica: in silico, in vitro, and in vivo Studies. Antioxidants. 2020; 9(2):103. Doi: https://doi.org/10.3390/antiox9020103 DOI: https://doi.org/10.3390/antiox9020103
Maslov OY., Kolisnyk SV., Komisarenko MA., Kostina TA., Dynnyk KV. Development the composition and technology for obtaining a dietary supplement “Cachinol” with the antioxidant activity in the form of granules used in the polycystic ovary syndrome. News Pharm. 2022; 103(1):42-7.
Doi: https://doi.org/10.24959/nphj.22.77 DOI: https://doi.org/10.24959/nphj.22.77
Drummond GR., Selemidis S., Griendling KK., Sobey CG. Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets. Nat Rev Drug Discov. 2011; 10(6):453-71.
Doi: https://doi.org/10.1038/nrd3403 DOI: https://doi.org/10.1038/nrd3403
Chikhale R., Sinha SK., Wanjari M., Gurav NS., Ayyanar M., Prasad S., Khanal P., Dey YN., Patil RB., Gurav SS. Computational assessment of saikosaponins as adjuvant treatment for COVID-19: molecular docking, dynamics, and network pharmacology analysis. Mol Divers. 2021; 25(3):1889-904.
Doi: https://doi.org/10.1007/s11030-021-10183-w DOI: https://doi.org/10.1007/s11030-021-10183-w
Maslov O., Kolesnik S., Komisarenko M., Altukhov A., Dynnyk K., Kostina T. Development and Validation of a Titrimetric Method for Quantitative Determination of Free Organic Acids in Green Tea Leaves. Pharmakeftiki. 2021; 33(4):304–11. Doi:
https://doi.org/10.5281/zenodo.7813135
Srivastava A., Greenspan P., Hartle DK., Hargrove JL., Amarowicz R., Pegg RB. Antioxidant and Anti-inflammatory Activities of Polyphenolics from Southeastern U.S. Range Blackberry Cultivars. J Agric Food Chem. 2010; 58(10):6102-9.
Doi: https://doi.org/10.1021/jf1004836 DOI: https://doi.org/10.1021/jf1004836
Velu V., Banerjee S., Rajendran V., Gupta G., Chellappan DK., Kumar N., Fuloria S., Mehta M., Dua K., Malipeddi H. Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties. AntiInflammatory Amp AntiAllergy Agents Med Chem. 2021; 20(3):308-315.
Doi: https://doi.org/10.2174/1871523020666210126144506 DOI: https://doi.org/10.2174/1871523020666210126144506