القياس الكمي للمانجيفيرين من كسر المنشط الحيوي من أوراق المانجو (منجيفيرا هندية ألـ.) وتقييم إمكانات التئام الجروح
DOI:
https://doi.org/10.35516/jjps.v16i3.652الكلمات المفتاحية:
كسر المنشط الحيوي، المنجفيرين، الحروق، الغشاء، القياس الكميالملخص
تشير الحروق إلى الأضرار التي تلحق بسطح الجلد بسبب ارتفاع درجات الحرارة من الزيت والماء والكهرباء والنار والتعرض لأشعة الشمس والمواد الكيميائية. تتطلب علاجا سريعا ومناسبا لتجنب الآثار غير المرغوب فيها. لذلك، تهدف هذه الدراسة إلى تحديد كمية المانجيفيرين، التي يمكن أن تعالج الحروق، في كسر المنشط الحيوي من أوراق المانجو (منجيفيرا هندية ألـ.) وتقييم نشاطها في التئام الحروق. تشمل الطرق المستخدمة في قياس إستشراب الطبقة الرقيقة (تي ألـ سي – (TLC - مكثافية بصرية مع التحقق من ضبط الخطية ، وحدود الكشف والقياس الكمي (ألـ أو دي LoD و ألـ أو قيو LoQ) والدقة والدقة والتقدير الكمي. تمت صياغة كسر المنشط الحيوي في غشاء بتركيز 5 / 10 و 15%. تم تطبيق الغشاء على الأرانب التي سبق أن تعرضت لحروق 6 جروح وعولجت ضد 4 ذكور من الأرانب. تم قياس تقدم الشفاء بقطر الحروق باستخدام المسماك المورن في كل 3 أيام إلى 21 يوما. تم الكشف عن المركب النشط (المانجيفيرين) بطول موجة 257 نانومتر. أنتجت نتائج الاختبار المعادلة الخطية واي = x76596 + 2935.7 مع قيمة معامل الارتباط 0.9957 ، حد الكشف 2.01 ميكروغرام / مل µg/mL، قيمة حد التقدير 6.07 ميكروغرام / مل، معامل الاختلاف في المدى 0.59 - 3.33 %، ومدى دقة 99.18 - 100.9% مع مستويات المانجيفيرين 208.31 ميكروغرام / مل. تم اختباراستعدادات غشاء كسر المنشط الحيوي المانجيفيرين على حروق من الدرجة الثانية في الأرانب. أظهرت النتائج أن التراكيز 10% و 15% كانت الأكثر فعالية.
المراجع
WHO, Burns. http://www.who.int/news-room/fact-sheets/detail/burns, 2018.
Departemen Kesehatan RI, Riset Kesehatan Dasar. Jakarta: Badan Penelitian dan pengembangan Kesehatan Kementrian Kesehatan RI, 2008.
Hidayat T.S., Noer S., Rizaliyana S., Peran Topikal Ekstrak Sel Aloe vera pada Penyembuhan Luka Bakar Derajat Dalam pada Tikus. Karya Akhir. Surabaya: Bagian Ilmu Bedah Plastik Rekonstruksi dan Estetik Fakultas Kedokteran Airlangga, 2013.
Gomez R., Murray C. K, Hospenthal D. R, Cancio L.C, Renz E.M, Holcomb J.B, Wade C.E, and Wolf S.E, Causes of Mortality by Autopsy Findings of Combat Casualties and Civilian Patients Admitted to Burn Unit. Journal of the American College of Surgeons. 2009; 208(3): 348-354.
Doi: 10.1016/j.jamcollsurg.2008.11.012 DOI: https://doi.org/10.1016/j.jamcollsurg.2008.11.012
Lachiewicz A. M, Hauck C. G, Weber D. J., and Cairns B. A., Bacterial Infections After Burn Injuries: Impact of Multidrug Resistance. Infectious Diseases Society of America. 2017; 65: 2130-2136. DOI: https://doi.org/10.1093/cid/cix682
Husnunnisa, Ismed F., Taher M., Ichwan S.J.A, Bakhtiar A, and Arbain D, Screening of Some Sumatran Medicinal Plants and Selected Secondary Metabolites for Their Cytotoxic Potential Against MCF-7 And HSC-3 Cell Lines. Journal of Research in Pharmacy, 2019; 23(4): 770-776. DOI: https://doi.org/10.12991/jrp.2019.186
Andania M. M., Ismed F., Taher M., Ichwan S.J.A, Bakhtiar A., and Arbain D., Cytotoxic Activities of Extracts and Isolated Compounds of Some Potential Sumatran Medicinal Plants against MCF-7 and HSC-3 Cell Lines, Journal of Mathematical and Fundamental Sciences. 2019; 51(3): 225-242
BPS. Produksi Buah Buahan, https://padangkota.bps.go.id/indicator/55/433/1/ produksi-buah-buahan.html, 2022.
Islam M, Mannan M, Kabir M.H.B, Islam A, and Olival K.J, Analgesic Antiinflammatory and Antimicrobial Effects of Etanol Extract of Mango Leaves. Journal of the Bangladesh Agricultural University. 2010; 8(2): 239-244. DOI: https://doi.org/10.3329/jbau.v8i2.7932
Derese S., Guantai E. M., Souaibou Y., and Kuete V., Mangifera indica L. (Anacardiaceae), in Medicinal Spices and Vegetables from Africa, Therapeutic Potential Against Metabolic, Inflammatory, Infectious and Systemic Diseases. 2017: 451-483,
https://doi.org/10.1016/B978-0-12-809286-6.00021-2 DOI: https://doi.org/10.1016/B978-0-12-809286-6.00021-2
Jung J.-S, Jung K, Kim N.-H, Kim H.-S, Selective Inhibition of MMP-9 Gene Expression by Mangiferin in PMA-Stimulated Human Astroglioma Cells: Involvement of PI3K/Akt and MAPK Signaling Pathways. Pharmacol. Res., 2012; 66: 95–103.
https://doi.org/10.1016/j.phrs.2012.02.013 DOI: https://doi.org/10.1016/j.phrs.2012.02.013
Kumar Y., Kumar V. Sangeeta, Comparative Antioxidant Capacity of Plant Leaves And Herbs With Their Antioxidative Potential In Meat System Under Accelerated Oxidation Conditions. J. Food Meas. Charact. 2020; 14: 3250–3262.
https://doi.org/10.1007/s11694-020-00571-5. DOI: https://doi.org/10.1007/s11694-020-00571-5
Doughari J. and Manzara S, In Vitro Antibacterial Activity of Crude Leaf Extracts of Mangifera indica Linn. African Journal of Microbiology Research, 2008; 2(1): 67-72.
Imran M., Arshad M. S., Butt M.S, Kwon J. H., Arshad M. U., and Sultan M. T., Mangiferin: A Natural Miracle Bioactive Compound Against Lifestyle Related Disorders, Lipids in Health and Disease, 2017; 16: 84. Doi: 10.1186/s12944-017-0449-y DOI: https://doi.org/10.1186/s12944-017-0449-y
Chandrappa C. P., Govindappa M., Kumar A.N.V., Channabasava R., Chandrasekar N., Umashankar T., and Mahabaleshwara K., Identification and Separation of Quercetin from Ethanol Extract of Carmona retusa By Thin Layer Chromatography and High-Performance Liquid Chromatography with Diode Array Detection. World Journal of Pharmacy and Pharmaceutical Sciences, 2014; 3(6).
Ferenczi-Fodor K., Renger B., and Végh Z., The Frustrated Reviewer—Recurrent Failures In Manuscripts Describing Validation of Quantitative TLC/HPTLC Procedures For Analysis of Pharmaceuticals. Journal of Planar Chromatography—Modern TLC. 2010, 23(3): 173-179. DOI: https://doi.org/10.1556/JPC.23.2010.3.1
Ismed F., Putra H. E., Arifa N., Putra D. P., Phytochemical profiling and antibacterial activities of extracts from five species of Sumatran lichen genus Stereocaulon. Jordan Journal of Pharmaceutical Sciences. 2021; 14(2).
Anggraeni J.V., Roni. A., and Yulianti S., Antioxidant Activity and Cytotoxic of N-Hexane and Methanol Extract from Mango Leaves (Mangifera indica L.). Indonesia Natural Research Pharmaceutical Journal. 2010; 5(2): 124-134 DOI: https://doi.org/10.52447/inspj.v5i2.4228
Rasyid R., Ruslan R., Mawaddah S., and Rivai H., Quantitative Determination of Mangiferin in Methanol Extract of Bacang Mango (Mangifera foetida L.) Leaves by Thin-Layer Chromatography Densitometry. World Journal of Pharmacy and Pharmaceutical Sciences. 2020; 9(7).
Tayana N., Inthakusol W., Duangdee S., Chewchinda S., Pandith H., and Kongkiatpaiboon S., Mangiferin Content in Different Parts of Mango Tree (Mangifera indica L.) in Thailand. Songklanakarin J Sci Technol. 2019; 41(3): 522-528.
Saroha K., Singh S., Aggarwal A., and Nanda S., Transdermal gels: An Alternative Vehicle for Drug Delivery. International Journal of Pharmaceutical, Chemical and Biological Sciences, 2013; 3(3): 495-503.
Kumar S.S., Behuy B., and Sachinkumar P., Formulation and Evaluation of Transdermal Patch of Stavudine. Dhaka University. Journal of Pharmaceutical Sciences. 2013; 12(1), 63-69. DOI: https://doi.org/10.3329/dujps.v12i1.16302
Lakshmi P.K., Pawana S., Rajpur A., and Prasanthi D., Formulation and Evaluation of Membrane-Controlled Transdermal Drug Delivery of Tolterodine Tartarate. Asian Journal of Pharmaceutical and Clinical Research, 2014; 7(2): 111-115.
Boddeda B., Suhasini M. S., Niranja P., Ramadevi M., and Anusha N., Design, Evaluation and Optimization of Fluconazole Transdermal Patch By 22 Factorial Method. Der Pharmacia Letter. 2016; 8(5): 280-287.
Hong J.W., Lee W.J., Hahn S.B., Kim B.J., Lew D.H, The Effect of Human Placenta Extract in a Wound Healing Model. Ann Plast Surg. 2010; 65: 96-100. DOI: https://doi.org/10.1097/SAP.0b013e3181b0bb67
Rowan M.P., Cancio L.C., Elster E.A., Burmeister D.M, Rose L.F, Natesan S, Chan R.K, Christy R.J, and Chung K.K, Burn Wound Healing and Treatment: Review and Advancements. Critical Care. 2015; 19(1), 243. DOI: https://doi.org/10.1186/s13054-015-0961-2
Said A., Wahid F., Bashir K., Rasheed H. M., Khan T., Hussain Z., and Siraj S., Sauromatum guttatum Extract Promotes Wound Healing and Tissue Regeneration in A Burn Mouse Model Via Up-Regulation of Growth Factors., Pharmaceutical Biology. 2019: 57(1): 736-743 DOI: https://doi.org/10.1080/13880209.2019.1676266
Anis A., Sharshar A., Hambally S. E., and Shehata A. A., Histopathological Evaluation of the Healing Process of Standardized Skin Burns in Rabbits: Assessment of a Natural Product with Honey and Essential Oils. Journal of Clinical Medicine. 2022; 11, 6417. DOI: https://doi.org/10.3390/jcm11216417
Contran RS., Kumar V., Collins T., Pathology Basic of Diseases (6th ed). 1999; Philadelphia: WB Saunders Co
Kumar H, Jain S and Shukla K, Evaluation of Alfalfa (Medicago sativa) Leaves for Wound Healing Activity. Journal of Pharmacognosy and Phytochemistry. 2020; 9(5): 1164-1169.
Tahir T., Bakri S., Patellongi I., Aman M., Miskad U., Yunis M., and Yusuf S., Effect of Hylocereus polyrhizus Extract to VEGF and TGF-β1 Level in Acute Wound Healing of Wistar Rats, Jordan Journal of Pharmaceutical Sciences, 2021; 14(1).
Du S., Liu H., Lei T., Xie X., Wang H., He X., Tong R., and Wang Y., Mangiferin: An Effective Therapeutic Agent Against Several Disorders (Review). Molecular Medicine Reports. 2018; 18: 4775-4786. DOI: https://doi.org/10.3892/mmr.2018.9529
Bulugonda R. K., Kumar K. A., Ganappa D., Beeda H., Philip G. H., Rao D. M., and Faisal S. M., Mangiferin from Pueraria tuberosa Reduces Inflammation Via Inactivation of NLRP3 Inflammasome. Scientific Reports, 2017; 7. DOI: https://doi.org/10.1038/srep42683
Mukherjee P. K., Verpoorte R., and Suresh B., Evaluation of In Vivo Wound Healing Activity of Hypericum patulum (Family: Hypericaceae) Leaf Extract on Different Wound Model in Rats. Journal of Ethnopharmacol. 2000; 70, 315-321. DOI: https://doi.org/10.1016/S0378-8741(99)00172-5
Swamya H.M.K., Krishna V., Shankarmurthy K., Rahiman B.A., Mankani K.L., Mahadevan K.M., Harish B.G., and Naika H.R., Wound Healing Activity of Embelin Isolated from The Ethanol Extract of Leaves of Embelia ribes Burm. Journal of Ethnopharmacol. 2007; 109, 29-34. DOI: https://doi.org/10.1016/j.jep.2006.09.003
