Antibiotic Resistance Patterns and Distribution of Extended- Spectrum Beta-Lactamases and Different Classes of Integrons Among Pseudomonas aeruginosa strains recovered from various clinical samples

Authors

  • Hamid Vaez Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
  • Mohsen Barani School of Medicine, Amiralmomenin hospital, Zabol University of Medical Sciences, Zabol, Iran
  • Mohsen Khamar School of Medicine, Amiralmomenin hospital, Zabol University of Medical Sciences, Zabol, Iran
  • Zahra Yazdanpour Department of Microbiology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran
  • Soudabeh Hamedi Shahraki Department of Epidemiology and Biostatistics, Faculty of Public Health, Zabol University of Medical Sciences, Zabol, Iran

DOI:

https://doi.org/10.35516/jmj.v59i1.2546

Keywords:

Pseudomonas aeruginosa, class A ESBL, multi-drug resistant, integrons

Abstract

Background: Treatment of infections caused by Pseudomonas
aeruginosa (P. aeruginosa) is becoming more difficult with each passing
year. Class A extended-spectrum beta-lactamases (ESBLs) and integrons
play a vital role in antibiotic treatment failure and ensuing poor patient
outcomes. We aimed to determine the prevalence of antibiotic resistance
patterns, class A ESBLs genes, as well as different classes of integrons
among P. aeruginosa strains obtained from clinical specimens.
methods: In total, 90 non-repetitive isolates of P.
aeruginosa were collected from clinical specimens. Standard
microbiology laboratory tests were used to identify P. aeruginosa.
Antibiotic resistance patterns were ascertained using the disc diffusion
method based on clinical and laboratory standard institute guidelines. The
PCR (polymerase chain reaction) was applied to detect ESBLs (blaCTXM,
blaSHV, and blaTEM) genes and different classes of integrons (I, II, and III).
Results: In this study, isolates were mostly resistant to ceftazidime 41
(45.6%) and gentamicin 39 (43.3%). Out of 90 investigated isolates, 25
(27.8%) were multi-drug resistant (MDR). ESBLs genes including
blaCTXM, blaTEM, and blaSHV were detected in 22.2% (20 of 90), 28.9% (26
of 90), and 31.1% (28 of 90) of the isolates, respectively. The prevalence
of class I and II integrons was 61.1% and 4.4%, respectively. Class III
integrons were not detected.
Conclusion: Based on this study's results, the prescription of ceftazidime
and gentamicin should be restricted. In addition, ESBLs genes and
integrons seem to play a significant role in the emergence and spread of
MDR infections. It is of pivotal importance that microbiology laboratory
remains vigilant about identifying ESBLs and integrons-positive isolates
through surveillance systems.

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Published

2025-02-27

How to Cite

Vaez, H., Barani, M., Khamar, M., Yazdanpour, Z., & Hamedi Shahraki, S. (2025). Antibiotic Resistance Patterns and Distribution of Extended- Spectrum Beta-Lactamases and Different Classes of Integrons Among Pseudomonas aeruginosa strains recovered from various clinical samples. Jordan Medical Journal, 59(1). https://doi.org/10.35516/jmj.v59i1.2546

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