Exploration of Antidiabetic and Diuretic Activities of Lumnitzera racemosa and Eclipta alba with Molecular Docking Study

Mrinal Mitra; Dilruba Jahan; Nihar Ronjon Uraon; Md. Abu Bakar Siddique; Pritam Kundu; Samir Kumar Sadhu; Utpal Kumar  Karmakar

doi:10.35516/jjps.v18i1.2752

Authors

Mrinal Mitra Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh
Dilruba Jahan Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh
Nihar Ronjon Uraon Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh
Md. Abu Bakar Siddique Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh
Pritam Kundu Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh
Samir Kumar Sadhu Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh
Utpal Kumar Karmakar Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh

DOI:

https://doi.org/10.35516/jjps.v18i1.2752

Keywords:

Lumnitzera racemosa, Eclipta alba, Diuretic activity, Antidiabetic activity, Molecular Docking Study

Abstract

The goal of the current study was to look at the leaves of Lumnitzera racemosa (Family: Combretaceae) and aerial part of Eclipta alba (Family: Asteraceae) for its phytochemical constituents and selected pharmacological activities (diuretic and antidiabetic). Diuretic medications are used to treat hypertension. Diabetes and hypertension are two common disease of geriatric patients and our aim was to explore medicinal plants of Sundarbans to find therapeutic of these diseases. The presence of carbohydrates, glycosides, reducing sugar, tannins, flavonoids, alkaloids, proteins, gum, steroids, saponin, and acidic chemicals was shown by phytochemical study of L. racemosa and E. alba showed the existence of tannin, saponin, flavonoid, gum, alkaloid, steroid, and terpenoid. Both plants extract was fractionated depending on polarity using n-hexane (non-polar), ethyl acetate (medium-polar) and water (polar). In the diuretic activity assay using Swiss Albino mice, none of the fractions of L. racemosa and E. alba showed diuretic activity but n-hexane 500 mg/kg of E. alba exhibited little diuretic activity compared to the standard frusemide. Swiss Albino mice were used to assess the oral glucose tolerance test (OGTT) in order to measure antidiabetic activity. In OGTT, water fraction 500 mg/kg of L. racemosa and E. alba showed blood glucose lowering activity compared to the standard Glibenclamide. We had performed in silico study among reported eight (08) compounds of L. racemosa against 1V4S (human glucokinase) protein. In comparison to classic glibenclamide (-8.5 kcal/mol), myricitrin had a good docking score of -8.4 kcal/mol. Based on these findings, we hypothesized that L. racemosa and E. alba could be a possible source of therapeutic leads for hypertension and hyperglycemia.

References

Manohar S.M. A Review of the Botany, Phytochemistry, and Pharmacology of Mangrove Lumnitzera racemosa Willd. Pharmacogn Rev. 2021; 15(30):107-116. DOI: 10.5530/phrev.2021.15.13. DOI: https://doi.org/10.5530/phrev.2021.15.13

Darwish A.G.G., Samy M.N., Sugimoto S., Otsuka H., Abdel-Salam H., Issa M.I., Shaker E.S., Matsunami K. Bioactive Compounds from the Leaves of Lumnitzera racemosa Against Acetaminophen-Induced Liver Damage in vitro. J. Arid Land Stud. 2016; 26(3):183-186. DOI: 10.14976/jals.26.3_183.

Molligoda S.P., Madushani G.H., Jayangani R.M.M., Hewageegana S.P. Pharmacological Activities of Eclipta alba (L.) Hassk. (Bhringaraja): A Review. GSC Adv. Res. Rev. 2023; 15(2):85-97.

DOI: 10.30574/gscarr.2023.15.2.0150. DOI: https://doi.org/10.30574/gscarr.2023.15.2.0150

Evans W.C. Trease and Evan's Textbook of Pharmacognosy. 13th ed. Cambridge University Press; 1989. p. 546.

Joy K.L., Kuttan R. Anti-Diabetic Activity of Picrorrhiza kurroa Extract. J. Ethnopharmacol. 1999; 67(2):143-148. DOI: 10.1016/s0378-8741(98)00243-8. DOI: https://doi.org/10.1016/S0378-8741(98)00243-8

Bnouham M., Merhfour F.Z., Ziyyat A., Mekhfi H., Aziz M., Legssyer A. Antihyperglycemic Activity of the Aqueous Extract of Urtica dioica. Fitoterapia. 2003; 74(7-8):677-681. DOI: 10.1016/s0367-326x(03)00182-5. DOI: https://doi.org/10.1016/S0367-326X(03)00182-5

Mamun M., Billah M., Ashek M., Ahasan M., Hossain M., Sultana T. Evaluation of Diuretic Activity of Ipomoea aquatica (Kalmisak) in Mice Model Study. J. Med. Sci. 2003; 3(5):395-400. DOI: 10.3923/jms.2003.395.400. DOI: https://doi.org/10.3923/jms.2003.395.400

Mekonnen T., Urga K., Engidawork E. Evaluation of the Diuretic and Analgesic Activities of the Rhizomes of Rumex abyssinicus Jacq in Mice. J. Ethnopharmacol. 2010; 127:433-439. DOI: 10.1016/j.jep.2009.10.020. DOI: https://doi.org/10.1016/j.jep.2009.10.020

Dallakyan S., Olson A.J. Small-Molecule Library Screening by Docking with PyRx. Methods Mol. Biol. 2015; 1263:243-250. DOI: 10.1007/978-1-4939-2269-7_19. DOI: https://doi.org/10.1007/978-1-4939-2269-7_19

Shaker B., Ahmad S., Lee J., Jung C., Na D. In Silico Methods and Tools for Drug Discovery. Comput. Biol. Med. 2021; 147:104851.

DOI: 10.1016/j.compbiomed.2021.104851. DOI: https://doi.org/10.1016/j.compbiomed.2021.104851

Yu S.Y., Wang S.W., Hwang T.L., Wei B.L., Su C.J., Chang F.R., Cheng Y.B. Components from the Leaves and Twigs of Mangrove Lumnitzera racemosa with Anti-Angiogenic and Anti-Inflammatory Effects. Mar. Drugs 2018; 16:404. DOI: 10.3390/md16110404. DOI: https://doi.org/10.3390/md16110404

Karmakar U.K., Akter S., Sultana S. Investigation of Antioxidant, Analgesic, Antimicrobial, and Anthelmintic Activity of the Aerial Parts of Paederia foetida (Family: Rubiaceae). Jordan J. Pharm. Sci. 2020; 13(2):131-147.

Sengupta R., Sheorey S.D., Hinge M.A. Analgesic and Anti-Inflammatory Plants: An Updated Review. Int. J. Pharm. Sci. Rev. Res. 2012; 12(2):114-119.

Kumar D., Kumar S., Singh J., Vashistha B., Singh N. Free Radical Scavenging and Analgesic Activities of Cucumis sativus L. Fruit Extract. J. Young Pharm. 2010; 2(4):365-368. DOI: 10.4103/0975-1483.71627. DOI: https://doi.org/10.4103/0975-1483.71627

El-Sayed A.M., Abdel-Ghani E.M., Tadros S.H., Soliman F.M. Pharmacognostical and Biological Exploration of Scaevola taccada (Gaertn.) Roxb. Grown in Egypt. Jordan J. Pharm. Sci. 2020; 13(4):435-455.

Ammal S.M.A., Sudha S., Rajkumar D., Baskaran A., Krishnamoorthy G., Anbumozhi M.K. In Silico Molecular Docking Studies of Phytocompounds from Coleus amboinicus Against Glucokinase. Cureus 2023; 15(2):e34507. DOI: 10.7759/cureus.34507. DOI: https://doi.org/10.7759/cureus.34507

Wilson J.E. Isozymes of Mammalian Hexokinase: Structure, Subcellular Localization, and Metabolic Function. J. Exp. Biol. 2003; 206:2049-2057. DOI: 10.1242/jeb.00241. DOI: https://doi.org/10.1242/jeb.00241

Ferre T., Riu E., Bosch F., Valera A. Evidence from Transgenic Mice That Glucokinase is Rate Limiting for Glucose Utilization in the Liver. FASEB J. 1996; 10:1213-1218. DOI: 10.1096/fasebj.10.10.8751724. DOI: https://doi.org/10.1096/fasebj.10.10.8751724

Karmakar U.K., Paul A., Kundu P., Paul P.P. Exploration of Anthelmintic, Blood Coagulant, Diuretic, and Laxative Activities of Different Solvent Fractions of Flagellaria indica Leaves. Jordan J. Pharm. Sci. 2023; 16(3):655-670. DOI: 10.35516/jjps.v16i3.976. DOI: https://doi.org/10.35516/jjps.v16i3.976

Al Disi S.S., Anwar M.A., Eid A.H. Anti-Hypertensive Herbs and Their Mechanisms of Action: Part I. Front. Pharmacol. 2016; 6:323.

DOI: 10.3389/fphar.2015.00323. DOI: https://doi.org/10.3389/fphar.2015.00323

Hock F.J. Drug Discovery and Evaluation: Pharmacological Assays. Springer; 2016:837–841. DOI: https://doi.org/10.1007/978-3-319-05392-9

Sam R., Ives H.E., Pearce D. Diuretic Agents. In: Basic and Clinical Pharmacology. McGraw Hill; 2018:254–274.

Cadwallader A.B., De La Torre X., Tieri A., Botrè F. The Abuse of Diuretics as Performance-Enhancing Drugs and Masking Agents in Sport Doping: Pharmacology, Toxicology, and Analysis. Br. J. Pharmacol. 2010; 161(1):1–16. DOI: 10.1111/j.1476-5381.2010.00789.x. DOI: https://doi.org/10.1111/j.1476-5381.2010.00789.x

Jackson E.K. Drugs Affecting Renal Excretory Function. In: Goodman & Gilman’s The Pharmacological Basis of Therapeutics. New York: McGraw Hill; 2018:445–470.

Mishra S., Sharma S.K., Yadav J., Kasana B. A Review on How Exactly Diuretic Drugs Work in Our Body. J. Drug Deliv. Ther. 2013; 3(5):115–120. DOI: https://doi.org/10.22270/jddt.v3i5.594

Smith H. Diuretics: A Review for the Pharmacist. S. Afr. Pharm. J. 2014; 81(7):18–21.