The Antioxidant, Antimicrobial, and Cytotoxic Properties of Garcinia mangostana L. Peel Extracts and Their Alpha-Mangostin Content
DOI:
https://doi.org/10.35516/jjps.v18i4.3157Keywords:
Garcinia mangostana, alpha-mangostin, solvent extraction, cytotoxicity, antioxidantAbstract
Garcinia mangostana L. (mangosteen) peel is widely used as a traditional medicine in Southeast Asia, known for its beneficial biological properties. This study assessed the optimal extraction solvent for a-mangostin from the peel of G. mangostana using 70 % ethanol, 70 % acetone, 80 % methanol, and water at room and boiling temperatures. Furthermore, the most optimal extract’s antioxidant and antimicrobial properties were studied. The 70% ethanol and 70% acetone solvents produced the highest a-mangostin recovery, but the 70% ethanol showed higher flavonoid and anthocyanin contents than 70% acetone. The ethanolic extract showed moderate antimicrobial activity against Escherichia coli and Staphylococcus aureus. The brine shrimp larvae toxicity assay showed that the organic solvents were toxic at 100 µg/mL. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that 400 μg/ml of 70 % ethanolic extract was toxic to the HepG2 cells. When challenged with tert-butyl hydroperoxide (t-BOOH)-induced oxidative stress, the ethanolic G. mangostana extract from concentrations 12.5 to 50 µg/mL showed protective effects against this oxidative stimulation. This study showed that the 70% ethanolic extract of G. mangostana peel showed optimal recovery of a-mangostin and other phytochemicals while showing protective effects against oxidative stimulations in HepG2 cells.
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