نباتُ الحمّاض التّفِه المنتشر برّياً في سوريا: تحليلٌ كيميائيٌّ نباتيّ وتقييمُ الفعاليّة المضادَّة للأكسدة في الزُّجاج لخلاصاتِ الأجزاءِ الهوائيّة والجذامير
DOI:
https://doi.org/10.35516/jjps.v17i4.2448الكلمات المفتاحية:
الحماض التفه Murr.، الفصيلة البطباطية، الفينولات، الأنتراكينونات، الفلافونوئيدات، الفعالية المضادة للأكسدةالملخص
استُخدِمَ نبات الحمّاض التَّفِه Rumex conglomeratus Murr. (من الفصيلة البطباطية Polygonaceae) على نطاقٍ واسع شعبيّاً لعلاجِ العديد من الأمراض مثل الأمراض الجلديّة، الالتهابات، الإمساك، والسّرطان. وتعود الاستخدامات الشعبيّة والفعاليات الحيوية بشكلٍ رئيسيّ لغنى نباتاتِ جنس الحمّاض بالمستقلباتِ الثّانويّة الفعّالةِ حيويّاً. تمثّل هذه الدِّراسة التقرير الأوّل للتّركيبِ الكيميائيّ والفعاليّة المضادّة للأكسدة للخلاصات المحضّرة من الأجزاء الهوائيّة والجذامير لنبات Rumex conglomeratus.. تمَّ في هذه الدّراسة تحضير الخلاصات المائيّة والإيثانوليّة، وأُجريت اختبارات الكشف الكيميائيّ الأوليّة، بالإضافة إلى تحديدِ إجمالي المحتوى من الفينولات، الفلافونوئيدات والأنتراكينونات، وتقييمِ الفعاليّة المضادّة للأكسدة باستخدام طرائق لونية يقاس فيها الامتصاص بجهاز UV-VIS spectrophotometer. أظهرت النّتائج أنّ R. conglomeratus مصدرٌ غنيٌّ بالمستقلباتِ الثّانويّة. وامتلكت خلاصةُ الجذامير الإيثانوليّة أعلى محتوى من الفينولات (502.55 ± 1.36 mg GAE / g DE) والأنتراكينونات (6.71 ± 0.106 mg RhE/g DE كما امتلكت أعلى فعاليّة كاسحة لجذور DPPH الحرّة (IC50 = 5.40 ± 0.380 mg/L)، وقدرةً إرجاعيّة في اختبارِ FRAP (0.230 ± 0.004 at 200 mg/L) واختبار القدرة الكلّيّة المضادّة للأكسدة (321.41 ± 6.94 mg AAE / g DE). وكانت الفعالية المضادّة للأكسدة مرتبطة بشكل قوي بكلٍّ من المحتوى الفينولي والأنتراكينوني. تشير هذه النّتائج إلى إمكانيّة استخدام خلاصات نبات الحمّاض التَّفِه كمضادّ أكسدةٍ قويّ، أو حتى كعاملٍ ملّيّن. ومع ذلك من الضّروريّ إجراء المزيد من الأبحاث المستقبليّة لتحرّي المأمونيّة والفعاليّة، مع التّأكيدِ على مواصلةِ الاستكشاف لعزل وتحديد المركّبات الكيميائيّةِ الفعّالة حيويّاً باستخدام طرائق الكروماتوغرافيا المختلفة، وتقييم العديد من الفعاليّات الحيويّة المُحتملة لخلاصات هذا النّبات الواعد.
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كيفية الاقتباس
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