تقييم السمية الجينية والقدرة المضاداة للأكسدة في مستخلصات أوراق عشبة الدم الشرقية في الفئران Ajuga orientalis L. (Lamiaceae)
DOI:
https://doi.org/10.35516/jjps.v17i4.2466الكلمات المفتاحية:
الأنوية الصغيرة، نبات الدم الشرقي، نبات طبي، إجمالي نشاط القدرة المضادة للأكسدةالملخص
يعد نبات عشبة الدم الشرقية من النباتات العطرية المستوطنة لمنطقة شرق البحر الابيض المتوسط والتي تستخدم بشكل شائع في الطب الشعبي في الاردن والدول المجاورة. على الرغم من استعمالها الواسع، إلا أنه لا توجد دراسات سمية على مستخلصات اوراقها. تهدف هذه الدراسة لتقييم سمية هذا النبات في مسخلصات الاوراق عن طريق الايثانول والماء لتقييم باستخدام اختبار تكوين الأنوية الصغيرة في كريات الدم الحمراء الصبغية السوية في الحمض النووي الريبوزي منقوص الأكسجين للفئران (DNA) وايضا لتقييم إجمالي نشاط القدرة المضادة للأكسدة. لتحديد الجرعة القاتلة الوسيطة (50LD) لمستخلصات أوراق نبات الدم الشرقي، تم اعطاء تراكيز متفاوته ل 66 من ذكور فأر Balb/c. تم حقن الفئران يوميا بداخل البطن بالتراكيز التالية 4000 و2000 و1000 و500 ملغ/كغ وتم استخدام 3 مجموعات كضوابط ولمدة 28 يوماً. تم فحص تكون الأنوية الصغيرة في كريات الدم الحمراء العادية، وتم تقييم عينات الكبد لقياس القدرة الكلية المضادة للأكسدة.كان تركيزالجرعة القاتلة الوسيطة لكلا المستخلصين 4000 ملغ/كغ، مع زيادة معنوية في تكوين الأنوية الصغيرة في كريات الدم الحمراء العادية كلما زاد تركيز الجرعة. اظهرت النتائج انخفاض في قيم إجمالي نشاط القدرة المضادة للأكسدة في كلا المستخلصين مقارنة بالضوابط. تشير النتائج إلى وجود سمية جينية لمستخلصات أوراق نبات الدم الشرقي في الفئران ، مما يحث على الحذر في استهلاكها من قبل البشر. يجب إجراء المزيد من الأبحاث لتقييم سلامتها وسميتها بشكل شامل، خاصةً مع استخدامها التقليدي الواسع في الطب الشعبي.
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