تحليل مختبري للتأثيرات المضادة للسرطان والسكري لمستخلص أوراق Teucrium orientale المحب للماء المزروع في منطقتين جغرافيتين في فلسطين
DOI:
https://doi.org/10.35516/jjps.v18i1.2492الكلمات المفتاحية:
Teucrium orientale، ألفا الأميليز، سرطان الرئة لويس، تكاثر الخلايا، موت الخلايا المبرمجالملخص
أظهرت العديد من الدراسات أن أنواع Teucrium orientale تمتلك خصائص علاجية مهمة، بما في ذلك النشاط المضاد للأكسدة، والمضاد للجراثيم، والمضاد للالتهابات، إضافةً إلى تأثيرها المزيل للتشنجات. تهدف هذه الدراسة إلى تقييم الأنشطة المحتملة المضادة لمرض السكري والسرطان لمستخلصات أوراقTeucrium orientale المحبة للماء، التي جُمعت من منطقتين جغرافيتين في فلسطين: القدس ورام الله. تم اختبار المستخلص المائي للنبات لتحديد تأثيره المضاد لمرض السكري عبر قياس تثبيط نشاط إنزيمα-amylase ، بينما تم تقييم تأثيره المضاد للسرطان على خلايا سرطان الرئة Lewis Lung (LLC) باستخدام قياس التدفق الخلوي لفحص تكاثر الخلايا، بالإضافة إلى تلطيخ Annexin-V / بروبيديوم يوديد (PI) للكشف عن موت الخلايا المبرمج. أظهر مستخلص Teucrium orientale من القدس قدرة تثبيطية قوية لإنزيمα-amylase بقيمة IC50 تبلغ 7.43 ± 0.84 ميكروغرام/مل، مقارنة بـ IC50 لمستخلص رام الله الذي بلغ 23.2 ± 0.29 ميكروغرام/مل. كانت هذه القيم أفضل من الشاهد الإيجابي Acarbose، الذي سجل IC50 = 43.91 ± 1.08 ميكروغرام/مل. في اختبار التأثير المضاد للسرطان، تم تعريض خلايا LLC لتركيزات مختلفة من مستخلصي T. orientale (0، 50، 100، 200، و400 ميكروغرام/مل) لمدة 24 ساعة، وتم قياس تكاثر الخلايا باستخدام اختبارXTT لوحظ التثبيط الكامل لنمو الخلايا عند 400 ميكروغرام/مل في كلا المستخلصين، إلا أن مستخلص القدس أظهر كفاءة أكبر في التثبيط عند التركيزات المنخفضة. من ناحية أخرى، لم تؤثر الزيادة في تراكيز المستخلصات من المنطقتين (50، 100، 200، و400 ميكروغرام/مل) على معدل موت الخلايا المبرمج في العينات الضابطة، بينما أدى العلاج بمستخلص Teucrium orientale إلى ارتفاع ملحوظ في معدل موت الخلايا المبرمج في خلاياLLC بجميع التراكيز المختبرة. وكان هذا التأثير مرتبطًا بزيادة علامة موت الخلايا المبرمج المتأخر Annexin-V+/PI+ علاوةً على ذلك، أظهر مستخلص القدس معدل موت خلايا مبرمج بلغ 90 ± 3.4% عند أعلى تركيز (400 ميكروغرام/مل)، مقارنة بـ 62.6 ± 3.4% لمستخلص رام الله، مما يشير إلى كفاءته الأعلى في تحفيز موت الخلايا المبرمج لخلايا LLC بناءً على هذه النتائج، تمتلك مستخلصاتTeucrium orientale إمكانات واعدة كعوامل طبيعية مضادة لمرض السكري والسرطان، نظرًا لقدرتها على تثبيط إنزيم α-amylase، وتقليل تكاثر الخلايا السرطانية، وتعزيز موتها المبرمج. إلا أن هناك حاجة إلى مزيد من الدراسات في النماذج الحية والتجارب قبل السريرية للتحقق من هذه التأثيرات.
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