Valerian and Hops Combination Versus Escitalopram in Models of Depression and Anxiety: A Cross-talk with Oxidative Stress

Authors

  • Omar Gammoh Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Yarmouk University, Jordan
  • Zaid Aburubaiha College of Health Sciences, American University, Jordan
  • Amal Mayyas College of Health Sciences, American University, Jordan
  • Walid Alkatib College of Health Sciences, American University, Jordan
  • Renad Masarweh College of Health Sciences, American University, Jordan
  • Feras Elhajji Faculty of Pharmacy, Applied Science Private University, Jordan
  • Abdelrahim Alqudah Department of Clinical Pharmacy and Pharmaceutical Practice, College of Pharmaceutical Sciences, Hashemite University, Jordan

DOI:

https://doi.org/10.35516/jjps.v16i1.1073

Keywords:

Antidepressants, anxiolytics, valerian-hops, mice model, stress

Abstract

Depression and anxiety disorders are the most common mental health problems and are associated with oxidative stress. Although famous for its anxiolytic effect, the antidepressant effect of the valerian-hops combination was not previously studied, also the relationship between the sedative effect of valerian-hops and oxidative stress markers is unclear. The current research has two objectives: (1) to compare the antidepressant effect of valerian-hops with escitalopram and (2) to evaluate the sedative/anxiolytic effects of valerian-hops in relation to oxidative stress markers namely Nitric Oxide (NOx), inducible Nitric Oxide Synthase (iNOS) and Super Oxide Dismutase (SOD). Two models were employed using BALB/c mice: A normal condition depression model in which mice were divided into: control, valerian-hops-treated (100mg/kg), and escitalopram-treated (10mg/kg) groups one hour before the open field test, the elevated plus-maze test, and the forced swim test and an anxiety model in which mice were divided into: unstressed naïve, control (stressed), valerian/hops (100mg/kg), and escitalopram (10 mg/kg) groups treated for three weeks; acutely restrained for 6 hours and sacrificed, serum was obtained to detect NOx, iNOS and SOD activity. In the depression model, valerian-hops demonstrated antidepressant activity similar to escitalopram (p>0.05). In the anxiety model, the valerian-hops treated mice demonstrated a profound sedative effect in all behavior paradigms (p<0.05), and normalized the anxiety-induced NOx levels and SOD activity (p<0.05). Under normal conditions, the valerian-hops combination exerts an antidepressant effect similar to escitalopram while in stress/anxiety conditions it exerts profound sedative and antioxidant effects.

Author Biographies

Omar Gammoh, Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Yarmouk University, Jordan

Department of Clinical Pharmacy and Pharmacy Practice, Faculty of Pharmacy, Yarmouk University, Jordan.

Zaid Aburubaiha, College of Health Sciences, American University, Jordan

College of Health Sciences, American University, Jordan.

Amal Mayyas, College of Health Sciences, American University, Jordan

College of Health Sciences, American University, Jordan.

Walid Alkatib, College of Health Sciences, American University, Jordan

College of Health Sciences, American University, Jordan.

Renad Masarweh, College of Health Sciences, American University, Jordan

College of Health Sciences, American University, Jordan.

Feras Elhajji, Faculty of Pharmacy, Applied Science Private University, Jordan

Faculty of Pharmacy, Applied Science Private University, Jordan.

Abdelrahim Alqudah, Department of Clinical Pharmacy and Pharmaceutical Practice, College of Pharmaceutical Sciences, Hashemite University, Jordan

Department of Clinical Pharmacy and Pharmaceutical Practice, College of Pharmaceutical Sciences, Hashemite University, Jordan.

References

Regehr C., Carey M., Wagner S., et al. Prevalence of PTSD, Depression and Anxiety Disorders in Correctional Officers : A Systematic Review Prevalence of PTSD , Depression and Anxiety Disorders in Correctional O ffi cers : A Systematic Review. Corrections [Internet]. 2019;0(0):1–13. Available from:

https://doi.org/10.1080/23774657.2019.1641765

Taher YA, Samud AM, Hashemi MM, et al. Prevalence of depression, anxiety and stress among Libyan primary and secondary Schoolteachers: a cross-sectional study. Jordan J. Pharm. Sci. 2016; 403(3972):1–12.

Hawgood J., De Leo D. Anxiety disorders and suicidal behavior: An update. Vol. 21, Current Opinion in Psychiatry. 2008. p. 51–64.

Hasin D.S., Sarvet A.L., Meyers J.L. et al. Epidemiology of Adult. 2018; 75(4):336–46.

Gammoh O., Mayyas F., Darwish Elhajji F. Chlorpheniramine and escitalopram: Similar antidepressant and nitric oxide lowering roles in a mouse model of anxiety. Biomed Reports. 2017 Jun;6(6):675–80.

Sowa-ku M., Stycze K., Siwek M., et al. Lipid Peroxidation and Immune Biomarkers Are Associated with Major Depression and Its Phenotypes, Including Treatment-Resistant Depression and Melancholia. 2018;448–60.

Gammoh OS, Al-Smadi A, Al-Awaida W.et al. Increased Salivary Nitric Oxide and G6PD Activity in Refugees with Anxiety and Stress. Stress Heal. 2016 Oct 1;32(4):435–40.

Jin L., Qin L., Xia D., et al. Active secretion and protective effect of salivary nitrate against stress in human volunteers and rats. Free Radic Biol Med. 2013 Apr;57:61–7.

Guix F.X., Uribesalgo I., Coma M., et al. The physiology and pathophysiology of nitric oxide in the brain. 2005;76:126–52.

Harkin A.J., Bruce K.H, Craft B, et al. Nitric oxide synthase inhibitors have antidepressant-like properties in mice 1 . Acute treatments are active in the forced swim test. 1999;207–13.

Blokhina O, Virolainen E, Fagerstedt K V. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot. 2003;91(2):179–94.

Rakesh G., Pae CU, Masand P.S. Beyond serotonin: newer antidepressants in the future. Expert Rev Neurother [Internet]. 2017;17(8): 777–90. Available from: http://dx.doi.org/10.1080/14737175.2017.1341310

Versus A.M.C.W., Papakostas G.I., Fava M., et al. Treatment of SSRI-Resistant Depression : Across-Class Switches. 2008; 0–5.

Unbehaun T, Spiegelhalder K, Hirscher V, et al. Nature and Science of Sleep Dovepress Management of insomnia: update and new approaches. Nat Sci Sleep [Internet]. 2010;2–127. Available from:

www.dovepress.com

Gammoh OS, Al-Smadi A, Turjman C, et al. Valerian: An underestimated anxiolytic in the community pharmacy? J Herb Med [Internet]. 2016;6(4):193–7. Available from:

http://dx.doi.org/10.1016/j.hermed.2016.09.001

Paul PP, Kundu P, Karmakar UK. Chemical and Biological Investigation of Sanchezia nobilis Leaves Extract. Jordan j. pharm. sci. 2022;15(1):121–31.

Patočka J, Jakl J. Biomedically relevant chemical constituents of Valeriana officinalis. J Appl Biomed. 2010;8(1):11–8.

Torkamani M.R.D, Abbaspour N., Jafari M., et al. Elicitation of valerenic acid in the hairy root cultures of Valeriana officinalis L (Valerianaceae). Trop J Pharm Res. 2014;13(6):943–9.

Attele A.S., Xie J.T., Yuan C.S. Treatment of insomnia: An alternative approach. Altern Med Rev. 2000; 5(3):249–59.

Becker A, Felgentreff F, Schröder H, et al. he anxiolytic effects of a Valerian extract is based on Valerenic acid. BMC Complement Altern Med. 2014;14:1–5.

Abourashed EA, Koetter U, Brattström A. In vitro binding experiments with valerian, hops and their fixed combination extract (Ze91019) to selected central nervous system receptors. Phytomedicine. 2004;11(7–8):633–8.

Yuan C, Mehendale S, Xiao Y, et al. The Gamma-Aminobutyric Acidergic Effects of Valerian and Valerenic Acid on Rat Brainstem Neuronal Activity. 2004.

Khom S., Khom S., Khom S., et al. Valerenic acid potentiates and inhibits GABA A receptors : Molecular mechanism and subunit specificity Related papers.

Dimpfel W., Brattström A., Koetter U. Central Action of A Fixed Valerian -Hops Extract Combination (Z E 91019) in freely moving rats. 2006;496–500.

Shah BN, Panchal MA, Gohil N, et al. PHYTO-PHARMACOLOGICAL PROFILE OF HUMULUS LUPULUS.

Wazaify M, Elayeh E, Tubeileh R, et al. Assessing insomnia management in community pharmacy setting in Jordan: A simulated patient approach. PLoS One. 2019;14(12):1–10.

Committee on Herbal Medicinal Products (HMPC). European Union herbal monograph on Valeriana officinalis L., flos. Eur Med Agency [Internet]. 2016; 31(February): 1–9. Available from:

https://www.ema.europa.eu/en/documents/herbal-monograph/final-european-union-herbal-monograph-valeriana-officinalis-l-radix_en.pdf

Hattesohl M., Feistel B., Sievers H., et al. Extracts of Valeriana officinalis L. s.l. show anxiolytic and antidepressant effects but neither sedative nor myorelaxant properties. Phytomedicine. 2008 Jan 25;15(1–2):2–15.

Sah S.P., Mathela C.S., Chopra K. Elucidation of possible mechanism of analgesic action of Valeriana Wallichii DC chemotype (patchouli alcohol) in experimental animal models. Indian J Exp Biol. 2010; 48(3):289–93.

Chow N.K., Fretz M., Hamburger M., et al. Telemetry as a tool to measure sedative effects of a valerian root extract and its single constituents in mice. Planta Med. 2011;77(8):795–803.

Machawal L., Kumar A. Possible involvement of nitric oxide mechanism in the neuroprotective effect of rutin against immobilization stress induced anxiety like behaviour, oxidative damage in mice. Pharmacol Reports. 2014;66(1):15–21.

Sah S.P., Mathela C.S., Chopra K. Involvement of nitric oxide (NO) signalling pathway in the antidepressant activity of essential oil of Valeriana wallichii Patchouli alcohol chemotype. Phytomedicine. 2011 Nov 15; 18(14): 1269–75.

Porsolt R.D., Bertin A, Blavet N., et al. Immobility induced by forced swimming in rats: Effects of agents which modify central catecholamine and serotonin activity. Eur J Pharmacol. 1979 Aug 1; 57(2–3):201–10.

Rodgers RJ, Dalvi A. Anxiety, defence and the elevated plus-maze. Neurosci & Biobehav Rev. 1997; 21(6):801–10.

Dishman R.K., Armstrong RB, Delp M.D., et al. Open-field behavior is not related to treadmill performance in exercising rats. Physiol Behav. 1988;43(5):541–6.

Green L.C., Wagner DA, Glogowski J, et al. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem. 1982;126(1):131–8.

Omori A, Yoshimura Y, Deyama Y, et al. Rosmarinic acid and arbutin suppress osteoclast differentiation by inhibiting superoxide and NFATc1 downregulation in RAW 264.7 cells. Biomed Reports. 2015 Jul;3(4):483–90.

Mag P, Kim JS, Ahn JD, et al. Effects of Valerianae Radix et Rhizoma extract on psychological stress in mice. 2015.

Franco L., Sánchez C., Bravo R., et al. The sedative effects of hops (Humulus lupulus), a component of beer, on the activity/rest rhythm. Acta Physiol Hung. 2012.

Vila-Verde C, Marinho ALZ, Lisboa SF et al. Nitric oxide in the prelimbic medial prefrontal cortex is involved in the anxiogenic-like effect induced by acute restraint stress in rats. Neuroscience. 2016 Apr 21;320:30–42.

Dyayiya N.A., Oyemitan IA, Matewu R., et al. Chemical analysis and biological potential of Valerian root as used by herbal practitioners in the Eastern Cape Province, South Africa. African J Tradit Complement Altern Med. 2016;13(1):114–22.

Kessing L.V., Hansen HV, Demyttenaere K., et al. Depressive and bipolar disorders: Patients’ attitudes and belief towards depression and antidepressants. Psychol Med. 2005 Aug;35(8):1205–13.

Wen X.J., Wang L.M., Liu Z.L., et al. Meta-analysis on the efficacy and tolerability of the augmentation of antidepressants with atypical antipsychotics in patients with major depressive disorder. Brazilian J Med Biol Res. 2014; 47(7):605–16.

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Published

2023-03-25

How to Cite

Gammoh, O. ., Aburubaiha, Z. ., Mayyas, A. ., Alkatib, W. ., Masarweh, R. ., Elhajji, F. ., & Alqudah, A. . (2023). Valerian and Hops Combination Versus Escitalopram in Models of Depression and Anxiety: A Cross-talk with Oxidative Stress. Jordan Journal of Pharmaceutical Sciences, 16(1), 124–136. https://doi.org/10.35516/jjps.v16i1.1073

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