Single-Nucleotide Polymorphism in Iraqi patients of TNF-α-308G/A(rs1800629) Susceptibility to Asthma
DOI:
https://doi.org/10.35516/jmj.v60i1.3601Keywords:
asthma, Iraqi patients, TNF-α-308G/A, susceptibility, polymorphism SSP-PCR techniqueAbstract
Objectives: The study examined the effects of genetic variations in TNF-α-308G/A, as well as TNF-α gene variants, using PCR-SSP to analyze individual characteristics.
Method: Researchers used molecular and immunological methods to investigate genotypes and alleles associated with asthma incidence, including 50 asthma patients and 40 healthy individuals.
Results: It was demonstrated that polymorphisms and the risk of developing asthma were correlated among asthma patients. The findings showed that, in comparison to the control group, asthma patients had considerably (P<0.05) greater levels of TNF-α-308G/A alleles than GA heterozygotes (TNF-α-308G/A).
Conclusion: Gene promoter polymorphisms may affect asthma sensitivity to TNF-α-308G/A, as evidenced by the strong association between the GG and AG gene genotypes and cytokine levels and disease development.
The development of asthma and immunological markers (TNF-α) are closely related. One perspective holds that there is a correlation between allergic rhinitis and the development and risk of asthma. It has been successful to use HDM immunotherapy to assist patients in establishing long-term clinical and immunological tolerance.
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