Germination Performance of Wild Lupine Seeds (Lupinus varius L.) in Jordan Using Different Breaking Dormancy Treatments

Wisam M. Obeidat; Khaldoun  O. AL SANE; Ahmad S. AL-EDWAN; Abdul Latief  AL-GHZAWI; Khaled M. ABULAILA; Mohammad  AL-SALEM; Abdul-salam  JUHMANI; Abdel Razzaq  ALTAWAHA

doi:10.35516/JJAS.3744

Authors

Wisam M. Obeidat University of Jordan, Jordan https://orcid.org/0009-0006-8261-8024
Khaldoun O. AL SANE National Agricultural Research Center (NARC), Jordan https://orcid.org/0000-0002-6394-096X
Ahmad S. AL-EDWAN National Agricultural Research Center (NARC), Jordan
Abdul Latief AL-GHZAWI The Hashemite University, Jordan https://orcid.org/0000-0003-2474-0620
Khaled M. ABULAILA National Agricultural Research Center (NARC), Jordan https://orcid.org/0000-0001-6746-4342
Mohammad AL-SALEM Jordan University of Science and Technology (JUST), Jordan https://orcid.org/0000-0002-0033-580X
Abdul-salam JUHMANI The Hashemite University, Jordan https://orcid.org/0000-0002-9904-3179
Abdel Razzaq ALTAWAHA National Agricultural Research Center (NARC), Jordan https://orcid.org/0000-0003-0826-0942

DOI:

https://doi.org/10.35516/JJAS.3744

Keywords:

Chemical scarification, germination rate, mechanical scarification, seed dormancy, radicle length

Abstract

Wild lupine (Lupinus varius L.) is an annual plant native to the Mediterranean region. Only a few studies have investigated the germination performance of wild lupine seeds in the Mediterranean region and examined different effects of temperatures and multiple dormancy-breaking treatments, which could help crop breeders to develop varieties more resilient to climate change. This study was conducted to assess how different dormancy-breaking treatments, including hot water (80 °C), chemical scarification using sulfuric acid (H₂SO₄), and mechanical scarification, affect the germination rate and radicle length of wild lupine seeds when exposed to four controlled temperatures: 10, 14, 18, and 22 °C. A complete randomized design with three replications was used in this study. Mechanical scarification combined with a temperature of 22°C for 15 days was the most effective treatment for breaking the dormancy and resulted in the highest germination percentage (93.3%) and the highest radicle length (7.6 mm) for lupine seeds. In contrast, the lowest germination percentage (13.1-13.3%) was observed for H₂SO₄ and control wild lupine seeds (untreated) incubated at 10°C. In conclusion, mechanical scarification is an excellent method for breaking the seed dormancy of wild lupine seeds.

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Author Biographies

Wisam M. Obeidat , University of Jordan, Jordan

University of Jordan, Faculty of Agriculture, Department of Plant Protection, Amman 11942, Jordan

Khaldoun O. AL SANE, National Agricultural Research Center (NARC), Jordan

National Agricultural Research Center (NARC), P.O. Box 639, Baq'a 19381 Jordan

Ahmad S. AL-EDWAN, National Agricultural Research Center (NARC), Jordan

National Agricultural Research Center (NARC), P.O. Box 639, Baq'a 19381 Jordan

Abdul Latief AL-GHZAWI, The Hashemite University, Jordan

The Hashemite University, Department of Biology and Biotechnology, P. O. Box 150459, Zarqa 13115 Jordan

Khaled M. ABULAILA, National Agricultural Research Center (NARC), Jordan

National Agricultural Research Center (NARC), P.O. Box 639, Baq'a 19381 Jordan.

Mohammad AL-SALEM, Jordan University of Science and Technology (JUST), Jordan

Jordan University of Science and Technology (JUST), Department of Plant Production, P.O.Box 3030, Irbid 22110 Jordan.

Abdul-salam JUHMANI, The Hashemite University, Jordan

The Hashemite University, Department of Biology and Biotechnology, P. O. Box 150459, Zarqa 13115 Jordan.

Abdel Razzaq ALTAWAHA, National Agricultural Research Center (NARC), Jordan

National Agricultural Research Center (NARC), P.O. Box 639, Baq'a 19381 Jordan.

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