Senna alata: Phytochemistry, Antioxidant, Thrombolytic, Anti-inflammatory, Cytotoxicity, Antibacterial activity, and GC-MS analysis

Deepa Karki; Bipindra  Pandey; Prabhat  Jha; Ashish Acharya; Dharma Prasad Khanal; Bechan Raut; Sandesh Panthi

doi:10.35516/jjps.v17i3.2406

Authors

Deepa Karki Manmohan Memorial Institute of Health Sciences, Tribhuvan University, Kathmandu, Nepal.
Bipindra Pandey Department of Pharmacy and Clinical Pharmacology, Madan Bhandari Academy of Health Sciences, Hetauda, Nepal.
Prabhat Jha School of Health and Allied Sciences, Pokhara University, Kaski, Nepal.
Ashish Acharya School of Health and Allied Sciences, Pokhara University, Kaski, Nepal.
Dharma Prasad Khanal Manmohan Memorial Institute of Health Sciences, Tribhuvan University, Kathmandu, Nepal.
Bechan Raut Manmohan Memorial Institute of Health Sciences, Tribhuvan University, Kathmandu, Nepal.
Sandesh Panthi PSN Education Pvt. Ltd., Gokarneshwor-5, Kathmandu 44600, Nepal

DOI:

https://doi.org/10.35516/jjps.v17i3.2406

Keywords:

Senna alata, phytochemical screening, antioxidant activity, anti-inflammatory activity, thrombolytic activity, cytotoxic activity

Abstract

Objective: Nepal's medicinal herbs are rich in cultural importance and have several uses. Senna alata, a plant belonging to the Leguminosae family, is prized for its aesthetic and therapeutic qualities. The goal of the study was to extract Senna alata leaves using several solvent macerations.

Methods: The study aims to evaluate the phytochemistry, total phenolic and flavonoid levels, antioxidant qualities in vitro, anti-inflammatory effects, cytotoxicity, anti-thrombolytic potential, and antibacterial activity, a variety of methodologies were employed.

Results: The extractive values of Senna alata were determined as 1.58%, 0.78%, and 5.92% in hexane, ethyl acetate, and methanol, respectively. GC-MS analysis revealed major compounds such as 3-Methylmannoside, Neophytadiene, Campesterol, and Vitamin E in the leaf extract. Qualitative phytochemical screening confirmed the presence of tannins, carbohydrates, flavonoids, cardiac glycosides, glycosides, and saponins in the methanol extract. The total phenolic and flavonoid contents were 46.36±4.5 mg GAE/g and 480.4±3.055 QE/g of dried extract, respectively. The extract exhibited significant antioxidant and anti-inflammatory activities, with IC50 values of 29.81 and 9.93, respectively. Additionally, it demonstrated cytotoxic activity with an LC50 value of 767.85 in the brine shrimp bioassay. In terms of thrombolytic activity, the extract showed clot lysis percentages of 7.89% and 10.13% at concentrations of 10 mg/ml and 25 mg/ml, respectively.

Conclusion: The methanolic extract of Senna alata leaves displayed therapeutic potential, including antioxidant, anti-inflammatory, cytotoxic, thrombolytic, and antibacterial effects. The presence of several bioactive compounds, as confirmed by GC-MS analysis, further supports the plant's potential for therapeutic use.

Author Biography

Bipindra Pandey, Department of Pharmacy and Clinical Pharmacology, Madan Bhandari Academy of Health Sciences, Hetauda, Nepal.

School of Health and Allied Sciences, Pokhara University, Kaski, Nepal.

References

Toh S.C., Lihan S., Bunya S.R., Leong S.S. In vitro antimicrobial efficacy of Cassia alata (Linn.) leaves, stem, and root extracts against cellulitis causative agent Staphylococcus aureus. BMC Complement Med Ther. 2023;23(1):023-03914. DOI: https://doi.org/10.1186/s12906-023-03914-z

Parveen A., Parveen B., Parveen R., Ahmad S. Challenges and guidelines for clinical trial of herbal drugs. J Pharm Bioallied Sci. 2015;7(4):329-33. DOI: https://doi.org/10.4103/0975-7406.168035

Sagnia B., Fedeli D., Casetti R., Montesano C., Falcioni G., Colizzi V. Antioxidant and anti-inflammatory activities of extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva medicinal plants collected in Cameroon. PLoS ONE. 2014;9(8). DOI: https://doi.org/10.1371/journal.pone.0103999

Carmona F., Pereira A.M.S. Herbal medicines: old and new concepts, truths and misunderstandings. Revista Brasileira de Farmacognosia. 2013;23(2):379-85. DOI: https://doi.org/10.1590/S0102-695X2013005000018

Arabshomali A., Bazzazzadehgan S., Mahdi F., Shariat-Madar Z. Potential benefits of antioxidant phytochemicals in type 2 diabetes. Molecules. 2023;28(20):7209. DOI: https://doi.org/10.3390/molecules28207209

Terao J. Potential role of quercetin glycosides as anti-atherosclerotic food-derived factors for human health. Antioxidants. 2023;12(2):258. DOI: https://doi.org/10.3390/antiox12020258

Laila O., Murtaza I., Muzamil S., Ali S.I., Ali S.A., Paray B.A., et al. Enhancement of nutraceutical and anti-diabetic potential of fenugreek (Trigonella foenum-graecum). Sprouts with natural elicitors. Saudi Pharmaceutical Journal. 2023;31(1):1-13. DOI: https://doi.org/10.1016/j.jsps.2022.11.001

Zhang Y.J., Gan R.Y., Li S., Zhou Y., Li A.N., Xu D.P., et al. Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules. 2015;20(12):21138-56. DOI: https://doi.org/10.3390/molecules201219753

Timothy S., Wazis C., Adati R., Maspalma I. Antifungal activity of aqueous and ethanolic leaf extracts of Cassia alata Linn. Journal of Applied Pharmaceutical Science. 2012;2(7):182-5. DOI: https://doi.org/10.7324/JAPS.2012.2728

Evans W.C. Trease & Evans Pharmacognosy (15Th Edition): Elsevier (A Divisionof Reed Elsevier India Pvt. Limited); 2002.

Yadav M., Chatterji S., Gupta S.K., Watal G. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. Int J Pharm Pharm Sci. 2014;6(5):539-42.

Yermeydan Peker M.g., Koç OmK, Üzer Ae, Apak Ra. Folin–Ciocalteu Reagent-Loaded Acrylamide-Based Hydrogel Sensor for Antioxidant Capacity Measurement with the Molybdenum Green Method. ACS Applied Polymer Materials. 2024;6(3):1864-77. DOI: https://doi.org/10.1021/acsapm.3c02712

Syukri Y., Purwati R., Hazami N., Tahmid H.A., Fitria A. Standardization of specific and non-specific parameters of propolis extract as raw material for herbal product. EKSAKTA: Journal of Sciences and Data Analysis. 2020 Feb 13:36-43. DOI: https://doi.org/10.20885/EKSAKTA.vol1.iss1.art6

Makuch E., Nowak A., Günther A. The effect of cream and gel vehicles on the percutaneous absorption and skin retention of a new eugenol derivative with antioxidant activity. Frontiers in Pharmacology. 2021 Jun 25;12:658381. DOI: https://doi.org/10.3389/fphar.2021.658381

Shodehinde S.A., Oboh G. Antioxidant properties of aqueous extracts of unripe Musa paradisiaca on sodium nitroprusside induced lipid peroxidation in rat pancreas in vitro. Asian Pac J Trop Biomed. 2013;3(6):449-57. DOI: https://doi.org/10.1016/S2221-1691(13)60095-7

Blois M.S. Antioxidant determinations by the use of a stable free radical. Nature. 1958;181(4617):1199-200. DOI: https://doi.org/10.1038/1811199a0

Mannan A., Kawser M.J., Ahmed A.A., Islam N.N., Alam S.M., Emon M.A.E.K., et al. Assessment of antibacterial, thrombolytic and cytotoxic potential of Cassia alata seed oil. Journal of Applied Pharmaceutical Science. 2011(Issue):56-9.

Panjaitan R.G., Yuliana Y.G. Description of acute toxicity of ketepeng root extract (Senna alata (L.) Roxb). Pharmacognosy Journal. 2022;14(4). DOI: https://doi.org/10.5530/pj.2022.14.113

Sedighinia F., Afshar A.S., Asili J., Ghazvini K. Antibacterial activity of Glycyrrhiza glabra against oral pathogens: an in vitro study. Avicenna Journal of Phytomedicine. 2012;2(3):118.

Kumar S., Pandey A.K. Chemistry and biological activities of flavonoids: an overview. ScientificWorldJournal. 2013;29(162750). DOI: https://doi.org/10.1155/2013/162750

Pamulaparthi A., Prathap V.R., Banala M., Nanna R.S. Total Phenolic, Flavonoid contents and Antioxidant assays in leaf extracts of Senna alata (L.) Roxb. Journal of Pharmaceutical Sciences and Research. 2016;8(9):981. DOI: https://doi.org/10.22159/ijcpr.2016v8i4.15280

Galanakis C., Goulas V., Tsakona S., Manganaris G., Gekas V. A knowledge base for the recovery of natural phenols with different solvents. Int J Food Prop. 2013;16:382-96. DOI: https://doi.org/10.1080/10942912.2010.522750

Russo D., Valentão P., Andrade P.B., Fernandez E.C., Milella L. Evaluation of Antioxidant, Antidiabetic and Anticholinesterase Activities of Smallanthus sonchifolius Landraces and Correlation with Their Phytochemical Profiles. Int J Mol Sci. 2015;16(8):17696-718. DOI: https://doi.org/10.3390/ijms160817696

Aklima J., Mojumder S., Sikdar D. Total phenolic content, reducing power, antioxidative and anti-amylase activities of five Bangladeshi fruits. International Food Research Journal. 2014;21(1):119.

Yadav R., Agrawal M. Phytochemical Analysis of Some Medicinal Plants. Journal of Phytology. 2011;3(12):10-4.

Kumar S., Mishra A., Pandey A.K. Antioxidant mediated protective effect of Parthenium hysterophorus against oxidative damage using in vitro models. BMC Complementary and Alternative Medicine. 2013;13(1):120. DOI: https://doi.org/10.1186/1472-6882-13-120

Singh R., Singh S., Kumar S., Arora S. Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn. Food Chem Toxicol. 2007;45(7):1216-23. DOI: https://doi.org/10.1016/j.fct.2007.01.002

Mishra A., Kumar S., Pandey A.K. Scientific validation of the medicinal efficacy of Tinospora cordifolia. ScientificWorldJournal. 2013;23(292934). DOI: https://doi.org/10.1155/2013/292934

Kumar S., Pandey A. Antioxidant, lipo-protective and antibacterial activities of phytoconstituents present in Solanum xanthocarpum root. International Review of Biophysical Chemistry. 2012;3(3):42-7.

Ogunjobi A., Abiala M. Antimicrobial activity of Senna alata and Phyllanthus amarus. Global Journal Of Pharmacology. 2013;7(2):198-202.

KC S., Pandey B., KC S., Gurung S., Gautam A. Anti-inflammatory, analgesic, and acute toxicity evaluation of Litchi chinensis seed extract in albino rat. Natural Resources for Human Health. 2021;1(1):30-5. DOI: https://doi.org/10.53365/nrfhh/141230

Pandey B., Thapa S., Kaundinnyayana A., Panta S. Hepatoprotective effects of Juglans regia on carbon tetrachloride‐induced hepatotoxicity: In silico/in vivo approach. Food Science & Nutrition. 2024. DOI: https://doi.org/10.1002/fsn3.4288

Pandey B., Baral R., Kaundinnyayana A., Panta S. Promising hepatoprotective agents from the natural sources: a study of scientific evidence. Egyptian Liver Journal. 2023;13(1):14. DOI: https://doi.org/10.1186/s43066-023-00248-w

El-Elimat T., Olimat S., Agha A.S.A., Aburjai A., Aburjai T. LC-MS Analysis of Secondary Metabolites of Asphodelus aestivus Brot.(Asphodelaceae) grown wild in Jordan. Jordan Journal of Pharmaceutical Sciences. 2024;17(2):333-43. DOI: https://doi.org/10.35516/jjps.v17i2.1850

Naskar A., Dasgupta A., Acharya K. Antioxidant and cytotoxic activity of Lentinus fasciatus. Jordan Journal of Pharmaceutical Sciences. 2023;16(1):72-81. DOI: https://doi.org/10.35516/jjps.v16i1.1064

Jemal K., Sandeep B., Pola S. Phytochemical screening and in vitro antioxidant activity analysis of leaf and callus extracts of Allophylus serratus (ROXB) KURZ. Jordan Journal of Pharmaceutical Sciences. 2022;15(1):51-69. DOI: https://doi.org/10.35516/jjps.v15i1.291