الفحص الكيميائي النباتي وتقييم التأثير المحسن لمستخلص أوراق نبات Aleuritopteris bicolor (Roxb.) Fraser.Jenk. على تلف الكلى والكبد في نموذج فئران يعاني من سمية كلوية مستحثة بالجنتاميسين
DOI:
https://doi.org/10.35516/jjps.v18i3.2534الكلمات المفتاحية:
Aleuritopteris bicolor، تحليل الكيمياء النباتية ، تأثيرات الحماية الكلوية ، تأثيرات الكبد ، سمية الجنتاميسينالملخص
الخلفية: أنواع السرخس aleuritopteris bicolor (AB) ، في عائلة Pteridaceae ، تستخدم تقليديًا لشفاء الجروح وعلاج أمراض مختلفة. الهدف: أجريت هذه الدراسة لتقييم إمكانية استخراج أوراق الإيثانول المائية (AB) في التخفيف من السمية الكلوية الناجمة عن الجنتاميسين والسمية الكبدية في الفئران البيضاء. الأساليب والمواد: تم تحقيق هذه الدراسة من خلال إجراء اختبار كيميائي نباتي على أوراق إيثانول AB المائية يستخلص وإدارة مستخلص الإيثانول المائي لأوراق AB شفهيًا وجنتاميسين (80 ملغ/كغ/يوم) داخل الوزراء ، و Liertains و Liver -eigh ، و Liver eigh ، يوم. تم استخدام SPSS الإصدار 23 لتحليل البيانات. تم تقديم النتائج على أنها متوسط الانحراف المعياري (ن = 6). تضمن التحليل الإحصائي ANOVA أحادي الاتجاه متبوعًا باختبار اختلاف أقل أهمية (LSD).
النتائج: من الفحص الكيميائي النباتي ، تم العثور على مستخلص الإيثانول المائي في aleuritopteris (AB) ليحتوي على الفلافونويد والفينولات والسابونين والعفص. أظهر اختبار السمية الحاد سلامته حتى 5000 ملغ/كغ. أسفرت إدارة الجنتاميسين (المجموعة II) عن زيادة كبيرة (P <0.001) في اليوريا (154.07 ± 6.22 ملغ/دل) ، الكرياتينين (8.90 ± 0.51 ملغ/دل) ، وحمض اليوريك (3.27 ± 0.74 ملغ/دل) ، مما يشير إلى مجموعة من العزف الكلوي. أدى المعالجة المشتركة مع حمض الأسكوربيك (المجموعة الثالثة) وجرعات متفاوتة من مستخلص أليوريتوبتيريس بيكولور (AB) (المجموعات IV و V و VI) إلى تخفيضات كبيرة في مستويات اليوريا والكرياتينين ، مع جرعة 500 ملغ/كغ من مستخلصات AB التي تظهر أكثر التأثيرات الملحوظة (P <0.001) مقارنةً بمجموعة Gentamicin-Inly. وكشف تحليل التشريح المرضي أن الجنتاميسين تسبب في تنكس أنبوبي ، وتشكيل المصبوب الغروي ، والإصابة الكبيبية ، في حين أن العلاج مع مستخلص AB يقلل من هذه الأضرار. بالإضافة إلى ذلك ، تسبب الجنتاميسين في زيادات كبيرة في ALP في المصل (P <0.001) ، AST (P <0.001) ، و ALT (P <0.001) مقارنة مع المجموعة الضابطة. العلاج مع مستخلص AB قلل بشكل كبير من هذه الإنزيمات (P <0.001) مقارنة مع مجموعة الجنتاميسين فقط. وأظهر التحليل النسيجي أن مجموعة الجنتاميسين كان لها التهاب في البوابة وانحطاط خلايا الكبد ، في حين أن مستخلص AB يقلل من هذه التغييرات ، مما يدعم آثارها الوقائية ضد السمية الكلوية والكبدية.
الاستنتاجات: تشير هذه النتائج إلى أن استخراج Aleuritopteris bicolor يخفف بشكل فعال من السمية الكلوية الناتجة عن الجنتاميسين والسمية الكبدية في الفئران البيضاء ، مما يدل على إمكانية الاستخدام العلاجي.
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