إمكانية التئام الجروح باستخدام الفوكويدان المستخرج بالموجات الدقيقة من نبات سارجاسوم وايتي، ربما بوساطة التعبير عن الكولاجين-1 في سلالة خلايا فيرو
DOI:
https://doi.org/10.35516/jjps.v18i3.2589الكلمات المفتاحية:
Sargassum wightii، wightiiFucoidan، جزيء كبير، التئام الجروح، اختبار SRB، خلايا Vero، التعبير عن الكولاجين-1الملخص
الخلفية: أظهر الفوكويدان، وهو جزيء طبيعي كبير مستخلص من نبات سارجاسوم وايتي، نتائج واعدة في مجالات علاجية متنوعة، بما في ذلك تطبيقات مضادة للأورام، ومضادة للأكسدة، ومضادة للتخثر، ومضادة للجروح. تستكشف هذه الدراسة إمكانات الفوكويدان المستخلص من نبات سارجاسوم وايتي، المُجمع من خليج مانار، تاميل نادو، الهند، في التئام الجروح.
الأهداف: يهدف هذا البحث إلى تقييم فعالية الفوكويدان المستخلص بتقنية الاستخلاص بمساعدة الميكروويف (MAE) في تعزيز التئام الجروح في خلايا فيرو، وهي سلالة من خلايا كلى القرد الأخضر الأفريقي. كما تبحث الدراسة في تأثير الفوكويدان على التعبير الجيني لبروتين الكولاجين-1، وهو بروتين أساسي يشارك في عملية التئام الجروح.
النتائج: أظهر اختبار SRB أن الفوكويدان لم يُظهر سمية خلوية لخلايا فيرو، حيث بلغت قيمة IC50 61.30 ميكرومولار. وكشف اختبار الخدش عن إغلاق جرح بنسبة 46.15% بعد 24 ساعة و76.9% بعد 48 ساعة، مقارنةً بـ 50% و81.25% في المجموعة الضابطة. وقد أدى علاج الفوكويدان إلى زيادة ملحوظة في التعبير عن الكولاجين-1، حيث أظهرت 77.92% من الخلايا مستويات مرتفعة من هذا البروتين الحيوي.
الخلاصة: تؤكد هذه الدراسة قدرة الفوكويدان المستخلص من نبات سارجاسوم وايتي على التئام الجروح في المختبر. وتدعم هذه النتائج إمكانات الفوكويدان كعامل طبيعي لالتئام الجروح وترميمها.
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