Investigation of Anticancer Activity of Lannea coromandelica Ethanol Bark Extract in 7, 12-Dimethylbenz (a) Anthracene Induced Cancer in Swiss Albino Mice

Prakash I. Nargatti; Kiran A. Wadkar

doi:10.35516/jjps.v19i1.2772

Authors

Prakash I. Nargatti Department of Pharmacology, Annasaheb Dange College of Pharmacy, Ashta, Sangli, Maharashtra- 416301 India; Research Scholar, Shri Appasaheb Birnale College of Pharmacy, Sangli, Maharashtra, 416416 India.
Kiran A. Wadkar Department of Pharmacognosy, Dr. Shivajirao Kadam College of Pharmacy, Kasbe Digraj, Sangli, Maharashtra, 416305 India

DOI:

https://doi.org/10.35516/jjps.v19i1.2772

Keywords:

Anticancer, histopathology, 5FU, Lannea coromandelica, MDA, SOD

Abstract

The present study was aimed with the focus of ethanol bark extract of Lannea coromandelica (LC) to assess an anticancer property in two stage carcinogenesis model using 7, 12-Dimethylbenz (a) anthracene (DMBA) and croton oil induced cancer in Swiss albino mice for 16 weeks. Extract of LC bark and Flonida 5% cream of 5-Fluorouracil (5-FU) as standard drug was applied topically every day for 16 weeks following DMBA application. The topical treatment of extract significantly reduces various tumour parameters. Effect of extract on Superoxide dismutase (SOD) and Catalase (CAT) level revealed that extract showed significant results (*p<0.05) as compared to standard group. The malondialdehyde (MDA) levels in the extract-treated group were nearly the same as those observed in the standard group, and both groups exhibited lower values than the normal control group. In addition, reduced glutathione (GSH) activity in the extract-treated group was increased compared with the carcinogen-treated control group. Histopathological alterations in carcinogen treated control group observed in the form of hyperplasia, dyskeratosis, and well-differentiated squamous cell carcinoma, presence of keratinized pearls, whereas these were found to be reduced after administration of extract and standard drug. An overall finding shows that LC bark extract has potent anticancer activity and provides a scientific basis for its chemopreventive property.

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